Sazhina T V, Ageeva T A
Arkh Patol. 2014 May-Jun;76(3):30-2.
To study the state of trophoblast and basal membranes of terminal placental villi in pregnant women with compensated diffuse toxic goiter.
Placental fragments from 38-40-week pregnant women were investigated by light microscopy and immunohistochemistry using PCNA, NCL-COLL-IV, and NCL-MMP-2-507 monoclonal antibodies ("Novocastra", United Kingdom).
Examination of the terminal placental villi revealed changes in their syncytiotrophoblast in the women with physiological and gestosis-complicated pregnancy and compensated diffuse toxic goiter; immunohistochemistry showed degradation of basal membranes and a change in their levels of type IV collagen.
In gestosis, compensatory mechanisms occur due to the larger number of symplastic buds and the numerical density of their nuclei and during hormonal imbalance due to the increase in the mean area of symplastic buds. Placental compensatory and adaptive mechanisms in pregnant women with diffuse toxic goiter call for further immunohistochemical investigation of the structural components of the placental barrier.
研究代偿性弥漫性毒性甲状腺肿孕妇胎盘终末绒毛的滋养层及基底膜状态。
采用PCNA、NCL-COLL-IV和NCL-MMP-2-507单克隆抗体（英国“诺华卡斯达”公司），通过光学显微镜和免疫组织化学方法对38 - 40周孕妇的胎盘组织碎片进行研究。
对胎盘终末绒毛的检查发现，生理妊娠、妊娠合并妊娠中毒症及代偿性弥漫性毒性甲状腺肿孕妇的合体滋养层均有变化；免疫组织化学显示基底膜降解及其IV型胶原水平改变。
在妊娠中毒症中，由于合体细胞芽数量增多及其核的数值密度增加，以及由于合体细胞芽平均面积增大导致激素失衡时，会出现代偿机制。弥漫性毒性甲状腺肿孕妇胎盘的代偿和适应机制需要对胎盘屏障的结构成分进行进一步的免疫组织化学研究。
