Boczoń K, Wandurska E, Imiłkowska K
Wiad Parazytol. 1989;35(5):435-48.
Changes in a bioenergetic state of Trichinella spiralis and Trichinella pseudospiralis infected rat and mouse muscle mitochondria were evaluated enzymatically, and in both infections 3-4-fold increase of mitochondrial, Mg++-stimulated ATP-ase (EC 3.6.1.3) was observed. Looking for the dynamics of those bioenergetic changes in T. pseudospiralis infected rat and mouse muscle mitochondria 1-2 weeks, pi, the 5-6-fold stimulation of mATP-ase activity, followed by a significant drop between the week 3-4th was found. In in vitro experiments cytoplasmic fractions isolated from T. spiralis and T. pseudospiralis larvae stimulated strongly mATP-ase activity of control rat liver mitochondria, the effect being much more pronounced in case of T. spiralis larvae. The factor(s) present in cytoplasmic fractions seem(s) to be heat-labile, of high molecular weight. Those experiments in vitro prove the causative role of the presence of T. spiralis and T. pseudospiralis larvae in the uncoupling of host muscle mitochondria. Since some relationship between the intensity of infection and the degree of uncoupling was observed, the measurements of the activity of this enzyme might serve not only as a biochemical method of differentiation between infected and normal muscles, but may be useful in crude evaluation of the intensity of these tissue infections.
通过酶学方法评估了感染旋毛虫和伪旋毛虫的大鼠和小鼠肌肉线粒体生物能状态的变化，在这两种感染中均观察到线粒体Mg++刺激的ATP酶（EC 3.6.1.3）增加了3至4倍。在感染伪旋毛虫的大鼠和小鼠肌肉线粒体中寻找这些生物能变化的动态，在感染后1至2周，发现mATP酶活性受到5至6倍的刺激，随后在第3至4周之间显著下降。在体外实验中，从旋毛虫和伪旋毛虫幼虫分离的细胞质部分强烈刺激了对照大鼠肝线粒体的mATP酶活性，在旋毛虫幼虫的情况下，这种作用更为明显。细胞质部分中存在的因子似乎对热不稳定，分子量较高。这些体外实验证明了旋毛虫和伪旋毛虫幼虫的存在对宿主肌肉线粒体解偶联的致病作用。由于观察到感染强度与解偶联程度之间存在某种关系，因此该酶活性的测量不仅可以作为区分感染肌肉和正常肌肉的生化方法，还可能有助于粗略评估这些组织感染的强度。
