Lenton L M, Behm C A, Bygrave F L
Division of Biochemistry and Molecular Biology, School of Life Sciences, Australian National University, Canberra.
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):425-31. doi: 10.1042/bj3070425.
The non-esterified fatty acid (NEFA) content and phospholipid composition of mitochondria isolated from the livers of Wistar rats infected with Fasciola hepatica were examined in relation to the aberrant mitochondrial respiration previously reported [Rule, Behm, and Bygrave (1989) Biochem. J. 260, 517-523]. At 2 weeks post-infection, elevated NEFA levels were associated with uncoupling of mitochondrial respiration that was reversible in vitro by the addition of BSA. State IV respiration rates showed a strong correlation with NEFA content. At 3 weeks post-infection, NEFA content had increased further and uncoupled mitochondria no longer showed any response to BSA. 31P-NMR analyses of cholate extracts of mitochondria from infected livers at 3 weeks post-infection revealed a marked loss of several major phospholipid species with a concomitant increase in catabolic products, particularly glycerophosphocholine and glycerophosphoethanolamine. Similar changes were observed in microsomal extracts. The NEFA content and phospholipid composition of mitochondria isolated from infected, athymic nude rats were not significantly different from uninfected, athymic rats. These findings suggest that uncoupling of liver mitochondria during infection with F. hepatica is the result of phospholipase activation mediated by the immune system of the host.
对感染肝片吸虫的Wistar大鼠肝脏中分离出的线粒体的非酯化脂肪酸（NEFA）含量和磷脂组成进行了检测，检测结果与之前报道的异常线粒体呼吸作用相关[Rule、Behm和Bygrave（1989年），《生物化学杂志》260卷，517 - 523页]。感染后2周，NEFA水平升高与线粒体呼吸解偶联有关，体外添加牛血清白蛋白（BSA）可使其恢复。状态IV呼吸速率与NEFA含量呈强相关。感染后3周，NEFA含量进一步增加，解偶联的线粒体对BSA不再有任何反应。对感染后3周的感染肝脏线粒体胆酸盐提取物进行的31P - NMR分析显示，几种主要磷脂种类显著减少，同时分解代谢产物增加，尤其是甘油磷酸胆碱和甘油磷酸乙醇胺。在微粒体提取物中也观察到类似变化。从感染的无胸腺裸鼠分离出的线粒体的NEFA含量和磷脂组成与未感染的无胸腺大鼠没有显著差异。这些发现表明，感染肝片吸虫期间肝脏线粒体的解偶联是宿主免疫系统介导的磷脂酶激活的结果。
