Zhao Shuai, Li Li, Li Shan-Hui, Wang Hong-Fei, Hozzein Wael N, Zhang Yong-Guang, Wadaan Mohammed A M, Li Wen-Jun, Tian Chang-Yan
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Jan;107(1):1-7. doi: 10.1007/s10482-014-0297-y. Epub 2014 Oct 12.
A Gram-stain-positive, aerobic, non-motile, coryneform bacterium, designated strain EGI 60002(T), was isolated from the halophyte Suaeda physophora. Cells were coryneform shaped and polymorphic. Phylogenetic analysis based on 16S rRNA gene sequences showed that the new isolate was closely related to Actinotalea ferrariae CF5-4(T) (95.8 % gene sequence similarity). The peptidoglycan type of strain EGI 60002(T) was A4β, containing L-Orn-D-Ser-D-Asp. The cell-wall sugars were mannose, ribose, rhamnose and glucose. The major fatty acids (>5 %) of strain EGI 60002(T) were iso-C14:0, iso-C15:0, anteiso-C15:1 A and anteiso-C15:0. The predominant respiratory quinone was MK-10(H4). The major polar lipids were diphosphatidylglycerol (DPG), one unidentified phosphoglycolipid (PGL) and one unidentified phospholipid (PL1). The genomic DNA G+C content was 72.3 mol%. On the basis of morphological, physiological, chemotaxonomic data, and phylogenetic analysis, strain EGI 60002(T) should be classified as a novel species within the genus Actinotalea, for which the name Actinotalea suaedae sp. nov. is proposed. The type strain is EGI 60002T (=JCM 19624(T) = KACC 17839(T) = KCTC 29256(T)).
从盐生植物囊果碱蓬中分离出一株革兰氏阳性、需氧、不运动的棒状杆菌，命名为菌株EGI 60002(T)。细胞呈棒状且多形性。基于16S rRNA基因序列的系统发育分析表明，该新分离菌株与铁氏放线盘菌CF5-4(T)密切相关（基因序列相似性为95.8%）。菌株EGI 60002(T)的肽聚糖类型为A4β，含有L-鸟氨酸-D-丝氨酸-D-天冬氨酸。细胞壁糖类为甘露糖、核糖、鼠李糖和葡萄糖。菌株EGI 60002(T)的主要脂肪酸（>5%）为异-C14:0、异-C15:0、anteiso-C15:1 A和anteiso-C15:0。主要呼吸醌为MK-10(H4)。主要极性脂为双磷脂酰甘油（DPG）、一种未鉴定的磷酸糖脂（PGL）和一种未鉴定的磷脂（PL1）。基因组DNA G+C含量为72.3 mol%。基于形态学、生理学、化学分类学数据和系统发育分析，菌株EGI 60002(T)应归类为放线盘菌属内的一个新物种，为此提出新名称囊果碱蓬放线盘菌（Actinotalea suaedae sp. nov.）。模式菌株为EGI 60002T（=JCM 19624(T)=KACC 17839(T)=KCTC 29256(T)）。
