Cho Hayoung, Hamada Moriyuki, Ahn Jae-Hyung, Weon Hang-Yeon, Joa Jae-Ho, Suzuki Ken-Ichiro, Kwon Soon-Wo, Kim Soo-Jin
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2017 Apr;67(3):704-709. doi: 10.1099/ijsem.0.001701. Epub 2017 Apr 3.
A bacterial strain, designated 5GHs33-3T, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. The strain was an aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. Strain 5GHs33-3T grew at 4-37 °C (optimally at 28-30 °C), pH 6.0-10.0 (optimally at pH 7.0) and with 0-7 % (w/v) NaCl (optimally with 0 %). The 16S rRNA gene sequence analysis revealed that strain 5GHs33-3T had high sequence similarity with Actinotalea suaedae EGI 60002T (98.4 %), Actinotalea ferrariae CF5-4T (96.4 %) and Actinotalea fermentans DSM 3133T (96.2 %), and less than 95.5 % sequence similarity against all the other species with validly published names. The phylogenetic tree revealed that strain 5GHs33-3T formed a robust independent monophyletic line with Actinotalea suaedae EGI 60002T. The predominant fatty acids of strain 5GHs33-3T were anteiso-C15 : 0 and iso-C14 : 0. The only quinone was MK-8(H4). Polar lipids were diphosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and unknown lipids. The peptidoglycan type was A4β, with ornithine as the diagnostic diamino acid and an interpeptide bridge comprising l-Glu. The DNA G+C content is 69.0 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain 5GHs33-3T represents a novel species of a new genus of the family Cellulomonadaceae, for which the name Pseudactinotalea terrae gen. nov., sp. nov. is proposed. The type strain of the type species is 5GHs33-3T (=KACC 16542T=NBRC 111006T). We also propose the reclassification of Actinotalea suaedae as Pseudactinotalea suaedae comb. nov. (type strain EGI 60002T=JCM 19624T=KACC 17839T=KCTC 29256T).
从韩国京畿道龙仁地区温室土壤中分离出一株细菌，命名为5GHs33-3T。该菌株为需氧型、革兰氏染色阳性、具鞭毛的杆状细菌。5GHs33-3T菌株在4-37 °C（最适温度为28-30 °C）、pH 6.0-10.0（最适pH为7.0）以及含0-7 %（w/v）NaCl（最适为0 %）的条件下生长。16S rRNA基因序列分析表明，5GHs33-3T菌株与碱蓬放线菌EGI 60002T（98.4 %）、铁色放线菌CF5-4T（96.4 %）和发酵放线菌DSM 3133T（96.2 %）具有较高的序列相似性，而与所有其他有效发表名称的物种的序列相似性均低于95.5 %。系统发育树显示，5GHs33-3T菌株与碱蓬放线菌EGI 60002T形成了一个稳健的独立单系分支。5GHs33-3T菌株的主要脂肪酸为anteiso-C15 : 0和iso-C14 : 0。唯一的醌为MK-8(H4)。极性脂为双磷脂酰甘油、磷脂酰肌醇、一种未知的磷酸糖脂和未知脂类。肽聚糖类型为A4β，以鸟氨酸作为诊断性二氨基酸，肽间桥由l-谷氨酸组成。DNA G+C含量为69.0 mol%。基于系统发育证据以及表型、基因型和化学分类分析结果，5GHs33-3T菌株代表纤维单胞菌科一个新属的一个新物种，为此提出新属名为假放线菌属（Pseudactinotalea），新种名为土壤假放线菌（Pseudactinotalea terrae）。模式种的模式菌株为5GHs33-3T（=KACC 16542T=NBRC 111006T）。我们还提议将碱蓬放线菌重新分类为碱蓬假放线菌（Pseudactinotalea suaedae）新组合（模式菌株EGI 60002T=JCM 19624T=KACC 17839T=KCTC 29256T）。
