Valizadeh Alireza, Bakhtiary Mohsen, Akbarzadeh Abolfazl, Salehi Roya, Frakhani Samad Mussa, Ebrahimi Ommolbanin, Rahmati-yamchi Mohammad, Davaran Soodabeh
a Department of Medical Nanotechnology , Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences , Tabriz , Iran.
b Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2016;44(2):504-9. doi: 10.3109/21691401.2014.965310. Epub 2014 Oct 13.
Nanofibrous scaffolds have many advantages that make them excellent candidates for tissue engineering applications. The scaffolds with high surface area to volume ratio favor cell adhesion, proliferation, migration and differentiation. In the present study, the preparation of electrospun poly (ϵ-caprolactone)-polyethylene glycol-poly (ϵ-caprolactone) (PCL-PEG-PCL) nanofibers is shown for the first time. PCL-PEG-PCL copolymers were synthesized using a ring-opening polymerization method. The polymers were characterized by FT-IR, (1)H NMR and DSC. Nanofibers with mean diameters ranging from 60 to 170 nm were obtained by the electrospinning method. Their morphology was evaluated by scanning electron microscopy (SEM). An MTT assay was used to compare the number of cells in the nanofiber scaffold. It was found that the morphology and diameter of the nanofiber depended on the chemical composition and molecular weight of the PEG segment of the copolymer used for electrospinning. Increasing the molecular weight of PEG blocks from 2000 to 6000 led to a decrease of the diameter of the fibers and the formation of beads.
纳米纤维支架具有许多优点，使其成为组织工程应用的理想候选材料。具有高表面积与体积比的支架有利于细胞粘附、增殖、迁移和分化。在本研究中，首次展示了电纺聚（ε-己内酯）-聚乙二醇-聚（ε-己内酯）（PCL-PEG-PCL）纳米纤维的制备。采用开环聚合法合成了PCL-PEG-PCL共聚物。通过傅里叶变换红外光谱（FT-IR）、核磁共振氢谱（¹H NMR）和差示扫描量热法（DSC）对聚合物进行了表征。通过电纺丝法获得了平均直径在60至170纳米范围内的纳米纤维。通过扫描电子显微镜（SEM）对其形态进行了评估。采用MTT法比较纳米纤维支架中的细胞数量。发现纳米纤维的形态和直径取决于用于电纺丝的共聚物中PEG链段的化学组成和分子量。将PEG嵌段的分子量从2000增加到6000导致纤维直径减小并形成珠粒。
