Hokmabad Vahideh Raeisdasteh, Davaran Soodabeh, Aghazadeh Marziyeh, Alizadeh Effat, Salehi Roya, Ramazani Ali
1Department of Chemistry, University of Zanjan, P.O. Box 45195-313, Zanjan, Iran.
2Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 51666-14733 Iran.
Tissue Eng Regen Med. 2018 Aug 14;15(6):735-750. doi: 10.1007/s13770-018-0140-z. eCollection 2018 Dec.
The major challenge of tissue engineering is to develop constructions with suitable properties which would mimic the natural extracellular matrix to induce the proliferation and differentiation of cells. Poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) (PCL-PEG-PCL, PCEC), chitosan (CS), nano-silica (n-SiO) and nano-hydroxyapatite (n-HA) are biomaterials successfully applied for the preparation of 3D structures appropriate for tissue engineering.
We evaluated the effect of n-HA and n-SiO incorporated PCEC-CS nanofibers on physical properties and osteogenic differentiation of human dental pulp stem cells (hDPSCs). Fourier transform infrared spectroscopy, field emission scanning electron microscope, transmission electron microscope, thermogravimetric analysis, contact angle and mechanical test were applied to evaluate the physicochemical properties of nanofibers. Cell adhesion and proliferation of hDPSCs and their osteoblastic differentiation on nanofibers were assessed using MTT assay, DAPI staining, alizarin red S staining, and QRT-PCR assay.
All the samples demonstrated bead-less morphologies with an average diameter in the range of 190-260 nm. The mechanical test studies showed that scaffolds incorporated with n-HA had a higher tensile strength than ones incorporated with n-SiO. While the hydrophilicity of n-SiO incorporated PCEC-CS nanofibers was higher than that of samples enriched with n-HA. Cell adhesion and proliferation studies showed that n-HA incorporated nanofibers were slightly superior to n-SiO incorporated ones. Alizarin red S staining and QRT-PCR analysis confirmed the osteogenic differentiation of hDPSCs on PCEC-CS nanofibers incorporated with n-HA and n-SiO.
Compared to other groups, PCEC-CS nanofibers incorporated with 15 wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications.
组织工程面临的主要挑战是开发具有合适特性的结构，以模拟天然细胞外基质，诱导细胞增殖和分化。聚（ε-己内酯）-聚（乙二醇）-聚（ε-己内酯）（PCL-PEG-PCL，PCEC）、壳聚糖（CS）、纳米二氧化硅（n-SiO）和纳米羟基磷灰石（n-HA）是成功应用于制备适合组织工程的三维结构的生物材料。
我们评估了掺入n-HA和n-SiO的PCEC-CS纳米纤维对人牙髓干细胞（hDPSCs）物理性质和成骨分化的影响。采用傅里叶变换红外光谱、场发射扫描电子显微镜、透射电子显微镜、热重分析、接触角和力学测试来评估纳米纤维的物理化学性质。使用MTT法、DAPI染色、茜素红S染色和QRT-PCR法评估hDPSCs在纳米纤维上的细胞黏附、增殖及其成骨细胞分化。
所有样品均呈现无珠状形态，平均直径在190-260nm范围内。力学测试研究表明，掺入n-HA的支架比掺入n-SiO的支架具有更高的拉伸强度。而掺入n-SiO的PCEC-CS纳米纤维的亲水性高于富含n-HA的样品。细胞黏附和增殖研究表明，掺入n-HA的纳米纤维略优于掺入n-SiO的纳米纤维。茜素红S染色和QRT-PCR分析证实了hDPSCs在掺入n-HA和n-SiO的PCEC-CS纳米纤维上的成骨分化。
与其他组相比，掺入15wt%n-HA的PCEC-CS纳米纤维能够支持更多的细胞黏附和分化，因此是骨组织工程应用的更好候选材料。
