小鼠原代神经元和星形胶质细胞中线粒体氧消耗率的测量。
Measurement of mitochondrial oxygen consumption rates in mouse primary neurons and astrocytes.
作者信息
Ribeiro Sofia M, Giménez-Cassina Alfredo, Danial Nika N
机构信息
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, 02115, USA.
出版信息
Methods Mol Biol. 2015;1241:59-69. doi: 10.1007/978-1-4939-1875-1_6.
The introduction of microplate-based assays that measure extracellular fluxes in intact, living cells has revolutionized the field of cellular bioenergetics. Here, we describe a method for real time assessment of mitochondrial oxygen consumption rates in primary mouse cortical neurons and astrocytes. This method requires the Extracellular Flux Analyzer Instrument (XF24, Seahorse Biosciences), which uses fluorescent oxygen sensors in a microplate assay format.
基于微孔板的检测方法可测量完整活细胞中的细胞外通量，该方法的引入彻底改变了细胞生物能量学领域。在此，我们描述一种实时评估原代小鼠皮质神经元和星形胶质细胞中线粒体氧消耗率的方法。此方法需要使用细胞外通量分析仪（XF24，安捷伦科技公司），该仪器在微孔板检测形式中使用荧光氧传感器。
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