Doherty Joanne R, Nilsson Lisa M, Kuliyev Emin, Zhu Haiqing, Matthew Rose, Cleveland John L, Mead Paul E, Roussel Martine F
Department of Pathology, St. Jude Children's Research Hospital, TN, USA.
Department of Biochemistry St. Jude Children's Research Hospital, TN, USA.
Cell Dev Biol. 2014 Feb 15;3(1). doi: 10.4172/2168-9296.1000133.
Here we report the cloning and functional characterization of the cyclin D-dependent kinase 4 and 6 (Cdk4/6) inhibitory protein Cdkn2d/p19 of (-Ink4d). - is the only family gene highly expressed during development and its transcripts were detected maternally and during neurulation. The -Ink4d protein has 63% identity to mouse and human Cdkn2d/p19 and its function as a negative regulator of cell cycle traverse is evolutionary conserved. Indeed, -lnk4d can functionally substitute for mouse Cdkn2d in binding to mouse Cdk4 and inhibiting cyclin-D1-dependent CDK4 kinase activity. Further, enforced expression of -lnk4d arrests mouse fibroblasts in the G1 phase of the cell cycle. These findings indicate that CDKN2d/p19 is conserved through vertebrate evolution and suggest -lnk4d may contribute to the development of .
在此，我们报告细胞周期蛋白D依赖性激酶4和6（Cdk4/6）抑制蛋白Cdkn2d/p19（-Ink4d）的克隆及功能特性。-是在发育过程中高度表达的唯一家族基因，其转录本在母体及神经胚形成过程中均可检测到。-Ink4d蛋白与小鼠和人类Cdkn2d/p19具有63%的同源性，其作为细胞周期进程负调控因子的功能在进化上是保守的。实际上，-lnk4d在结合小鼠Cdk4并抑制细胞周期蛋白D1依赖性CDK4激酶活性方面可在功能上替代小鼠Cdkn2d。此外，-lnk4d的强制表达使小鼠成纤维细胞停滞在细胞周期的G1期。这些发现表明CDKN2d/p19在脊椎动物进化过程中是保守的，并提示-lnk4d可能有助于[此处原文缺失具体内容]的发育。
