Laczka Olivier F, Labbate Maurizio, Seymour Justin R, Bourne David G, Fielder Stewart S, Doblin Martina A
Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia.
The ithree institute, University of Technology, Sydney, NSW, Australia.
PLoS One. 2014 Oct 13;9(10):e108387. doi: 10.1371/journal.pone.0108387. eCollection 2014.
Bacteria from the genus Vibrio are a common and environmentally important group of bacteria within coastal environments and include species pathogenic to aquaculture organisms. Their distribution and abundance are linked to specific environmental parameters, including temperature, salinity and nutrient enrichment. Accurate and efficient detection of Vibrios in environmental samples provides a potential important indicator of overall ecosystem health while also allowing rapid management responses for species pathogenic to humans or species implicated in disease of economically important aquacultured fish and invertebrates. In this study, we developed a surface immuno-functionalisation protocol, based on an avidin-biotin type covalent binding strategy, allowing specific sandwich-type detection of bacteria from the Vibrio genus. The assay was optimized on 12 diverse Vibrio strains, including species that have implications for aquaculture industries, reaching detection limits between 7×10(3) to 3×10(4) cells mL(-1). Current techniques for the detection of total Vibrios rely on laborious or inefficient analyses resulting in delayed management decisions. This work represents a novel approach for a rapid, accurate, sensitive and robust tool for quantifying Vibrios directly in industrial systems and in the environment, thereby facilitating rapid management responses.
弧菌属细菌是沿海环境中常见且对环境具有重要意义的一类细菌，其中包括对水产养殖生物致病的物种。它们的分布和丰度与特定的环境参数相关，包括温度、盐度和营养物质富集情况。准确、高效地检测环境样本中的弧菌，不仅能为整个生态系统健康状况提供一个潜在的重要指标，还能针对对人类致病的物种或与经济上重要的水产养殖鱼类和无脊椎动物疾病有关的物种迅速做出管理应对措施。在本研究中，我们基于抗生物素蛋白-生物素型共价结合策略开发了一种表面免疫功能化方案，可实现对弧菌属细菌的特异性夹心型检测。该检测方法在12种不同的弧菌菌株上进行了优化，包括对水产养殖业有影响的物种，检测限达到7×10³至3×10⁴个细胞/毫升。目前检测总弧菌的技术依赖于费力或低效的分析，导致管理决策延迟。这项工作代表了一种新颖的方法，可用于快速、准确、灵敏且稳健地直接在工业系统和环境中对弧菌进行定量，从而促进快速的管理应对。
