State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), 130 Meilong Road, Shanghai 200237, PR China.
Bioresour Technol. 2014 Dec;173:324-333. doi: 10.1016/j.biortech.2014.09.096. Epub 2014 Sep 28.
In this study, the culture medium components screening and filtering were undertaken in order to set up efficient and cost effective minimal culture media for lipid production from Yarrowia lipolytica DSM3286. The basal minimal culture medium (S2) designed yielded lipid content up to 35% of the microbial dry cell weight. A set of fermentation strategies based on this minimal medium was developed and the lipid content was raised to 51%. The scale-up under different fermentation conditions based on S2 medium led to a maximum lipid content of 65%. The produced microbial oils displayed interesting properties to be used as a feedstock for high quality biodiesel production. The minimal media and operable cultivation strategies devised in this study, in association with the works done so far by other authors, could enable fast, massive, viable and more economical production of single cell oils and smooth biodiesel manufacture.
在这项研究中，进行了培养基成分的筛选和过滤，以便为从解脂耶氏酵母 DSM3286 生产油脂建立高效、经济的最小培养基。设计的基础最小培养基 (S2) 使微生物干重的脂质含量高达 35%。基于该最小培养基开发了一系列发酵策略，将脂质含量提高到 51%。根据 S2 培养基在不同发酵条件下的放大，脂质含量最高可达 65%。所生产的微生物油具有作为高质量生物柴油生产原料的有趣特性。本研究设计的最小培养基和可操作的培养策略，以及迄今为止其他作者的工作，可以实现单细胞油的快速、大规模、可行和更经济的生产，以及生物柴油的顺利制造。
