Arshi Saba, Babaie Delara, Nabavi Mohammad, Tebianian Majid, Ghalehbaghi Babak, Jalali Farhad, Ahmadvand Alireza, Gholami Reza
Department of Allergy and Clinical Immunology, Hazrate Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran.
Int J Dermatol. 2014 Dec;53(12):e561-6. doi: 10.1111/ijd.12630. Epub 2014 Oct 14.
CD4+ CD25+ T-regulatory (Treg) cells play critical roles in maintaining peripheral tolerance and preventing autoimmunity. As characteristics of Treg cells have not been precisely investigated in chronic urticaria (CU) yet, this study was performed.
To determine the frequencies of circulating CD4+ CD25+ FOXP3+ T cells and serum levels of interleukin (IL)-10, transforming growth factor (TGF)-β, and IL-17 in patients with chronic autoimmune urticaria and chronic idiopathic urticaria compared to healthy controls.
Peripheral blood mononuclear cells (PBMCs) were obtained from patients with CU and healthy controls. The frequency of CD4+ CD25+ T cells in PBMCs and expression levels of FOXP3 were detected by flow cytometry. The serum levels of IL-10, TGF-β, and IL-17 were measured by enzyme-linked immunosorbent assay.
A significant decrease in the percentage of circulating CD4+ CD25+ FOXP3+ T cells was detected in patients with CU, compared to control subjects. However, no significant difference was detected on the serum levels of IL-10, TGF-β, and IL-17 between patients with CU and control subjects.
This study demonstrated that the frequency of Treg cells in PBMCs was decreased in patients with CU. Further studies are needed to clarify the exact role of Treg cells in the pathogenesis of CU and factors regulating their function.
CD4+ CD25+ 调节性T（Treg）细胞在维持外周免疫耐受和预防自身免疫中起关键作用。由于尚未在慢性荨麻疹（CU）中精确研究Treg细胞的特征，因此开展了本研究。
与健康对照相比，确定慢性自身免疫性荨麻疹和慢性特发性荨麻疹患者循环中CD4+ CD25+ FOXP3+ T细胞的频率以及白细胞介素（IL）-10、转化生长因子（TGF）-β和IL-17的血清水平。
从慢性荨麻疹患者和健康对照中获取外周血单个核细胞（PBMC）。通过流式细胞术检测PBMC中CD4+ CD25+ T细胞的频率和FOXP3的表达水平。采用酶联免疫吸附测定法测量IL-10、TGF-β和IL-17的血清水平。
与对照受试者相比，慢性荨麻疹患者循环中CD4+ CD25+ FOXP3+ T细胞百分比显著降低。然而，慢性荨麻疹患者与对照受试者之间IL-10、TGF-β和IL-17的血清水平未检测到显著差异。
本研究表明，慢性荨麻疹患者PBMC中Treg细胞频率降低。需要进一步研究以阐明Treg细胞在慢性荨麻疹发病机制中的确切作用以及调节其功能的因素。
