Li Jingyuan, Zhang Xiaoyu, Huang Haiying
Department of Infectious Diseases, The Second Affiliated Hospital of Harbin Medical University, Harbin 150000, China.
Department of Infectious Diseases, The Second Affiliated Hospital of Harbin Medical University, Harbin 150000, China.
Int Immunopharmacol. 2014 Dec;23(2):523-9. doi: 10.1016/j.intimp.2014.10.001. Epub 2014 Oct 12.
Linalool, a natural compound of the essential oils, has been shown to have antinociceptive, antimicrobial, and anti-inflammatory properties. The aim of this study was to investigate the effects of linalool against lipopolysaccharide (LPS)/D-galactosamine (GalN)-induced liver injury in mice. Mice were administered with linalool 1h before receiving LPS (50 μg/kg) and GalN (800 mg/kg). The results demonstrated that linalool had a protective effect on LPS/GalN-induced acute liver injury, as evidenced by the attenuation of hepatic pathological damage, malondialdehyde (MDA) content, MPO activity and serum ALT and AST levels. Linalool alleviated serum and hepatic TNF-α and IL-6 production, as well as hepatic iNOS and COX-2 expression by inhibiting NF-κB activation. Treatment of linalool increased bcl-2 expression and inhibited caspase-3 and caspase-8 expression. In addition, linalool increased Nrf2 and heme oxygenase-1 expression up-regulation by LPS/GalN. In conclusion, our results suggested that linalool was protected against LPS/GalN-induced liver injury through induction of antioxidant defense via Nrf2 activating and reduction inflammatory response via NF-κB inhibition.
芳樟醇是一种精油中的天然化合物，已被证明具有抗伤害感受、抗菌和抗炎特性。本研究的目的是探讨芳樟醇对脂多糖（LPS）/D-半乳糖胺（GalN）诱导的小鼠肝损伤的影响。在给予LPS（50μg/kg）和GalN（800mg/kg）前1小时给小鼠施用芳樟醇。结果表明，芳樟醇对LPS/GalN诱导的急性肝损伤具有保护作用，肝病理损伤减轻、丙二醛（MDA）含量、髓过氧化物酶（MPO）活性以及血清谷丙转氨酶（ALT）和谷草转氨酶（AST）水平降低证明了这一点。芳樟醇通过抑制核因子κB（NF-κB）激活减轻血清和肝脏肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的产生，以及肝脏诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的表达。芳樟醇治疗增加了bcl-2表达并抑制了半胱天冬酶-3（caspase-3）和半胱天冬酶-8（caspase-8）表达。此外，芳樟醇增加了LPS/GalN诱导的核因子E2相关因子2（Nrf2）和血红素加氧酶-1（HO-1）表达上调。总之，我们的结果表明，芳樟醇通过激活Nrf2诱导抗氧化防御和抑制NF-κB减轻炎症反应来保护免受LPS/GalN诱导的肝损伤。
