从黎巴嫩生长的月桂中提取的多糖、精油和脂肪油的抗菌及抗生物膜活性。

Antibacterial and antibiofilm activities of polysaccharides, essential oil, and fatty oil extracted from Laurus nobilis growing in Lebanon.

作者信息

Chmit Mohammad, Kanaan Hussein, Habib Jean, Abbass Mustafa, Mcheik Ali, Chokr Ali

机构信息

PRASE, Platform of Research and Analysis in Environmental Sciences, Doctoral School of Sciences and Technologies, Lebanese University, PO Box 5, Hadath Campus, Beirut, Lebanon.

Laboratory of Biotechnology of Natural Substances and Health Products, Faculty of Pharmacy, Lebanese University, Beirut, Lebanon.

出版信息

Asian Pac J Trop Med. 2014 Sep;7S1:S546-52. doi: 10.1016/S1995-7645(14)60288-1.

DOI:10.1016/S1995-7645(14)60288-1

PMID:25312182

Abstract

OBJECTIVE

To evaluate the antibacterial activity of the extracts of Laurus nobilis against three Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Staphylococcus epidermidis CIP 444) and two Gram-negative bacteria (Escherichia coli ATCC 35218 and Pseudomonas aeruginosa ATCC 27853). Also, the antibiofilm activity has been investigated against the biofilm produced by Staphylococcus epidermidis CIP 444.

MATERIALS

The polysaccharides, essential oil, and fatty oils extracted from the plant were used in broth microdilution methods to study the minimal inhibitory concentration, and then the minimal bactericidal concentration was determined.

RESULTS

The results showed that alginate, fucoidan, fatty oils and essential oil have good antibacterial activities against the 5 bacterial strains, and a negligible biofilm eradication activity of fucoidan, laminaran, fatty oil, and essential oil was observed, but a promising biofilm eradication activity was obtained with alginate, which showed a reduced biofilm mass even at low concentration.

CONCLUSIONS

The extracts obtained have promising antibacterial capacities which need further investigation for them to be incorporated in medical or nutritional applications.

摘要

目的

评估月桂叶提取物对三种革兰氏阳性菌（金黄色葡萄球菌ATCC 25923、粪肠球菌ATCC 29212和表皮葡萄球菌CIP 444）以及两种革兰氏阴性菌（大肠杆菌ATCC 35218和铜绿假单胞菌ATCC 27853）的抗菌活性。此外，还研究了其对表皮葡萄球菌CIP 444产生的生物膜的抗生物膜活性。