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1
A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells.一种定义明确的无动物来源和非饲养层的培养体系，用于衍生、扩增和直接分化无转基因的患者特异性诱导多能干细胞。
Biomaterials. 2014 Mar;35(9):2816-26. doi: 10.1016/j.biomaterials.2013.12.050. Epub 2014 Jan 9.
2
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.一种新型高效无饲养层培养体系，用于诱导人多能干细胞的衍生。
Sci Rep. 2014 Jan 8;4:3594. doi: 10.1038/srep03594.
3
Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings.简明综述：人类多能干细胞培养的演进：从饲养细胞到合成涂层。
Stem Cells. 2013 Jan;31(1):1-7. doi: 10.1002/stem.1260.
4
Derivation of mesenchymal stem cells from human induced pluripotent stem cells cultured on synthetic substrates.在合成基底上培养的人诱导多能干细胞衍生的间充质干细胞。
Stem Cells. 2012 Jun;30(6):1174-81. doi: 10.1002/stem.1084.
5
Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.筛选多种族来源的人类胚胎干细胞，鉴定出具有生长优势的染色体 20 最小扩增子。
Nat Biotechnol. 2011 Nov 27;29(12):1132-44. doi: 10.1038/nbt.2051.
6
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.合成聚合物涂层的制备及其在人胚胎干细胞无饲养层培养中的应用。
Nat Protoc. 2011 Jun 23;6(7):1037-43. doi: 10.1038/nprot.2011.342.
7
Chemically defined conditions for human iPSC derivation and culture.人诱导多能干细胞的化学定义条件及其培养。
Nat Methods. 2011 May;8(5):424-9. doi: 10.1038/nmeth.1593. Epub 2011 Apr 10.
8
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.利用合成修饰 mRNA 高效重编程人类细胞为多能性干细胞并进行定向分化。
Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
9
Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum.在重组E-钙黏蛋白基质上使用完全确定的条件培养人多能干细胞。
BMC Dev Biol. 2010 Jun 2;10:60. doi: 10.1186/1471-213X-10-60.
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High resolution array-CGH characterization of human stem cells using a stem cell focused microarray.使用干细胞专用微阵列对人类干细胞进行高分辨率 array-CGH 分析。
Mol Biotechnol. 2010 Nov;46(3):234-42. doi: 10.1007/s12033-010-9294-1.

在合成基质上无转基因人类诱导多能干细胞的衍生与长期培养。

Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.

作者信息

Villa-Diaz Luis Gerardo, Kim Jin Koo, Lahann Joerg, Krebsbach Paul H

机构信息

Department of Biologic and Materials Sciences, Biointerfaces Institute, and Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA.

Department of Biologic and Materials Sciences, Biointerfaces Institute, and Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA

出版信息

Stem Cells Transl Med. 2014 Dec;3(12):1410-7. doi: 10.5966/sctm.2014-0087. Epub 2014 Oct 13.

DOI:10.5966/sctm.2014-0087
PMID:25313201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4250210/
Abstract

We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.

摘要

我们描述了一个平台，用于在由接枝两性离子水凝胶聚2-（甲基丙烯酰氧基）乙基二甲基-（3-磺丙基）氢氧化铵（PMEDSAH）制成的全合成聚合物基质上衍生、培养和分化基因组稳定、无转基因的人类诱导多能干细胞（iPSC）。在这些条件下衍生的三个独立的无转基因iPSC系在PMEDSAH接枝平板上连续体外培养长达9个月后，在体外和体内均表现出持续的自我更新、基因组稳定性和多能性。总之，这些数据证明了这个替代平台在为再生医学生成和维持人类iPSC方面所具有的优势。