在合成基质上无转基因人类诱导多能干细胞的衍生与长期培养。
Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates.
作者信息
Villa-Diaz Luis Gerardo, Kim Jin Koo, Lahann Joerg, Krebsbach Paul H
机构信息
Department of Biologic and Materials Sciences, Biointerfaces Institute, and Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Department of Biologic and Materials Sciences, Biointerfaces Institute, and Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA
出版信息
Stem Cells Transl Med. 2014 Dec;3(12):1410-7. doi: 10.5966/sctm.2014-0087. Epub 2014 Oct 13.
Abstract
We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.
摘要
我们描述了一个平台，用于在由接枝两性离子水凝胶聚2-（甲基丙烯酰氧基）乙基二甲基-（3-磺丙基）氢氧化铵（PMEDSAH）制成的全合成聚合物基质上衍生、培养和分化基因组稳定、无转基因的人类诱导多能干细胞（iPSC）。在这些条件下衍生的三个独立的无转基因iPSC系在PMEDSAH接枝平板上连续体外培养长达9个月后，在体外和体内均表现出持续的自我更新、基因组稳定性和多能性。总之，这些数据证明了这个替代平台在为再生医学生成和维持人类iPSC方面所具有的优势。
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