Freitas Michelle A R, Alvarenga Ângela C, Fernandes Helen C, Gil Frederico F, Melo Maria N, Pesquero Jorge L, Gomes Maria A
Laboratório de Parasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Campus Umuarama, Uberlandia, MG, Brazil.
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antonio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Biomed Res Int. 2014;2014:285607. doi: 10.1155/2014/285607. Epub 2014 Sep 17.
Entamoeba histolytica is a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridization (SSH) technique, differential gene expressions of two E. histolytica strains, one virulent (EGG) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be involved with amoebic virulence. Nine cDNA fragments presenting high homology with E. histolytica previously sequenced genes were subtracted. Of these, four genes were confirmed by RT-PCR. Two coding for hypothetical proteins, one for a cysteine-rich protein, expressed only in the virulent strain, EGG and another one, coding for grainin 2 protein, exclusive from 452 strain. This study provided new insight into the proteins differences in the virulent and nonvirulent E. histolytica strains. We believe that further studies with these proteins may prove association of them with tissue damage, providing new perceptions to improve treatment or diagnosis of the invasive disease.
溶组织内阿米巴是一种具有降解组织能力的寄生虫，因此具有致病行为。由于并非所有菌株都表现出这种行为，所以已经有多项研究探讨细胞毒性过程的分子基础。利用抑制性消减杂交（SSH）技术，分析了两种溶组织内阿米巴菌株（一种有毒力的EGG和一种无毒力的452）的差异基因表达，目的是分离出可能与阿米巴毒力有关的基因。九个与溶组织内阿米巴先前测序基因具有高度同源性的cDNA片段被消减。其中，四个基因通过逆转录聚合酶链反应（RT-PCR）得到证实。两个编码假定蛋白，一个编码富含半胱氨酸的蛋白，仅在有毒力的EGG菌株中表达，另一个编码谷蛋白2，仅在452菌株中存在。这项研究为有毒力和无毒力的溶组织内阿米巴菌株之间的蛋白质差异提供了新的见解。我们相信，对这些蛋白质的进一步研究可能会证明它们与组织损伤有关，为改善侵袭性疾病的治疗或诊断提供新的认识。
