Allred Laura K, Sealey Voyksner Jennifer A, Voyksner Robert D
J AOAC Int. 2014 Nov-Dec;97(6):1615-25. doi: 10.5740/jaoacint.14-058.
To meet the need for the detection and quantitation of barley gluten in beer, qualitative screening and quantitative immunoassays based on the monoclonal antigluten antibody 401/21 (Skerritt) were validated in a single laboratory. Sample replicates were tested at each stage of beer production using multiple yeast strains and methods of end-stage protein removal. Quantitation was performed using barley-specific standards based on barley flour extracts. Immunoassay results were confirmed using LC/MS/MS for barley-specific peptides. The LOD for the qualitative screening test was 5 mg/L barley gluten. Recovery for the barley-spiked worts ranged from 81 to 128% in the quantitative ELISA assay; the LOD was <1 mg/L, and the LOQ was 5 mg/L. Both screening and confirmation methods were found to be fit for the purposes of detection of low levels of barley gluten in beer.
为满足啤酒中大麦谷蛋白检测和定量的需求，基于单克隆抗谷蛋白抗体401/21（斯克里特）的定性筛选和定量免疫测定在单一实验室中得到验证。在啤酒生产的每个阶段，使用多种酵母菌株和终末阶段蛋白质去除方法对样品复制品进行测试。使用基于大麦粉提取物的大麦特异性标准品进行定量。使用LC/MS/MS对大麦特异性肽段进行测定以确认免疫测定结果。定性筛选试验的检测限为5 mg/L大麦谷蛋白。在定量ELISA测定中，添加大麦的麦芽汁回收率在81%至128%之间；检测限<1 mg/L，定量限为5 mg/L。筛选和确证方法均适用于检测啤酒中低水平的大麦谷蛋白。
