Marbella C O, Gaafar S M
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
Vet Parasitol. 1989 Nov;34(1-2):63-70. doi: 10.1016/0304-4017(89)90165-9.
Pigs of 10 days-1 month old were orally infected with eggs of Ascaris suum at different rates and inoculation schedules. Histological sections from various parts of the small intestines were prepared to observe the production and localization of immunoglobulin-bearing cells. Fluorescent antibody and immunoperoxidase staining methods were used to determine the number of IgM-, IgA- and IgG-producing plasma cells in the intestinal lamina propria. Significant increases in immunoglobulin-bearing cells were observed in those pigs which received single inoculations of A. suum eggs. Pigs infected every 2,4,8 and 10 days with 10,000-20,000 embryonated eggs showed numerical increases in IgM-bearing cells. Increases in IgA-bearing cells were noted in pigs which received the higher number of eggs every 8-10 days. Higher concentrations of IgA- and IgM-bearing cells were observed in the jejunal mucosa of infected pigs as compared to those in the duodenum and ileum.
选用10日龄至1月龄的仔猪，按照不同的感染率和接种方案经口感染猪蛔虫卵。制备小肠各部位的组织切片，以观察含免疫球蛋白细胞的产生及定位情况。采用荧光抗体和免疫过氧化物酶染色方法，测定肠固有层中产生IgM、IgA和IgG的浆细胞数量。在单次接种猪蛔虫卵的仔猪中，观察到含免疫球蛋白细胞显著增加。每隔2、4、8和10天用10,000 - 20,000枚感染性虫卵感染的仔猪，其含IgM细胞数量增加。每隔8 - 10天接种较多虫卵的仔猪，其含IgA细胞数量增加。与十二指肠和回肠相比，在感染仔猪的空肠黏膜中观察到更高浓度的含IgA和IgM细胞。
