Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, 26 Sklabinská St., 036 01, Martin, Slovakia,
Adv Exp Med Biol. 2015;838:27-34. doi: 10.1007/5584_2014_75.
Our previous studies show that provinol, a polyphenolic compound, has anti-inflammatory activity during allergic inflammation. In the present study we investigated the effects of provinol and its combinations with clinically used antiasthmatics: budesonide or theophylline on airway defense mechanisms during experimental allergic asthma. Separate groups of guinea pigs were treated during the course of 21-day ovalbumin sensitization with provinol (20 mg/kg/day, p.o.), or budesonide (1 mM by inhalation), or theophylline (10 mg/kg/day, i.p.), and with a half-dose combination of provinol+budesonide or provinol+theophylline. Airways defense mechanisms: cough reflex and specific airway resistance (sRaw) were evaluated in vivo. Tracheal smooth muscle reactivity and mucociliary clearance were examined in vitro. The findings were that provinol caused significant decreases in sRaw and in tracheal smooth muscle contractility, a suppression of cough reflex, and positively modulated ciliary beat frequency. The bronchodilatory and antitussive effects of provinol were comparable with those of budesonide and theophylline. Provinol given as add-on treatment significantly potentiated the effects of budesonide or theophylline, although the doses of each were halved. We conclude that provinol not only has bronchodilatory and antitussive effects, but also potentiates similar effects exerted by budesonide and theophylline.
我们之前的研究表明，多酚化合物普罗醇在过敏炎症期间具有抗炎活性。在本研究中，我们研究了普罗醇及其与临床使用的抗哮喘药：布地奈德或茶碱的组合对实验性过敏性哮喘期间气道防御机制的影响。在 21 天卵清蛋白致敏期间，单独的豚鼠组用普罗醇（20mg/kg/天，po）、布地奈德（1mM 吸入）或茶碱（10mg/kg/天，ip）治疗，并以普罗醇+布地奈德或普罗醇+茶碱的半剂量组合治疗。气道防御机制：体内评估咳嗽反射和特定气道阻力（sRaw）。体外检查气管平滑肌反应性和黏液纤毛清除率。结果表明，普罗醇导致 sRaw 和气管平滑肌收缩显著降低，咳嗽反射受到抑制，并正向调节纤毛摆动频率。普罗醇的支气管扩张和镇咳作用与布地奈德和茶碱相当。作为附加治疗的普罗醇显著增强了布地奈德或茶碱的作用，尽管每种药物的剂量减半。我们得出结论，普罗醇不仅具有支气管扩张和镇咳作用，而且还增强了布地奈德和茶碱的类似作用。
