Department of Pharmacology, Comenius University, Martin, Slovakia.
Adv Exp Med Biol. 2013;756:91-8. doi: 10.1007/978-94-007-4549-0_12.
The aim of the study was to investigate the potential anti-inflammatory effects in -experimental allergic asthma of natural polyphenolic compounds or their single major components. The experiment was performed after 21-days sensitization of guinea pigs with ovalbumin suspension. Changes in airway reactivity after the long-term treatment with the polyphenolic compounds Provinol and Flavin-7 and their single major components quercetin and resveratrol during were assessed using a whole body plethysmography. Reactivity of tracheal smooth muscle was studied in vitro in response to cumulative doses of the bronchoconstrictive mediators histamine and acetylcholine. Furthermore, concentrations of the inflammatory cytokines IL-4 and IL-5 were measured in bronchoalveolar lavage fluid. The results demonstrate significant anti-inflammatory effects of Provinol and Flavin-7 exerted in the airways. In contrast, chronic treatment with quercetin and resveratrol, single components of the two polyphenols, did not show such activity. We conclude that polyphenolic compounds are more effective in the anti-inflammatory effects in the airways than their separate components.
本研究旨在探讨天然多酚化合物或其单一主要成分在实验性变应性哮喘中的潜在抗炎作用。该实验在豚鼠用卵清蛋白悬浮液致敏 21 天后进行。使用全身体积描记法评估长期使用多酚化合物 Provinol 和 Flavin-7 及其单一主要成分槲皮素和白藜芦醇对气道反应性的影响。在体外研究了气管平滑肌对累积剂量的支气管收缩介质组胺和乙酰胆碱的反应性。此外，还测量了支气管肺泡灌洗液中炎症细胞因子 IL-4 和 IL-5 的浓度。结果表明，Provinol 和 Flavin-7 在气道中具有显著的抗炎作用。相比之下，两种多酚的单一成分槲皮素和白藜芦醇的慢性治疗并未显示出这种活性。我们得出结论，多酚化合物在气道中的抗炎作用比其单独的成分更有效。
