Department of Gastroenterological Surgery, National Kyushu Cancer Center, 3-1-1 Notame, Minami-ku, Fukuoka, 811-1395, Japan,
Cancer Metastasis Rev. 2014 Dec;33(4):837-42. doi: 10.1007/s10555-014-9510-8.
Metastasis is a complex series of sequential events involving several gene products and the regulated expression of several tumor cell genes. Using rat mammary adenocarcinoma cell lines of differing metastatic potentials and a differential complementary DNA (cDNA) hybridization method, our laboratory embarked in 1992 on a project to identify candidate metastasis-associated genes. Among the genes that were found to be abundantly overexpressed in highly metastatic rat cell lines compared to poorly metastatic cell lines, we identified a completely novel gene without any homologous or related genes in the database in 1994. The full-length cDNA of this gene was cloned, sequenced, and named mta1 (metastasis-associated gene 1), and eventually, its human cDNA counterpart, MTA1, was also cloned and sequenced by our group. MTA1 has now been identified as one of the members of a gene family (MTA gene family). The products of the MTA genes, the MTA proteins, are transcriptional co-regulators that function in histone deacetylation and nucleosome remodeling. In this review, we will briefly discuss the researches for the identification and characterization of the mta1 gene, its human counterpart MTA1, and their protein products.
转移是一个复杂的连续事件系列，涉及多个基因产物和几个肿瘤细胞基因的调节表达。我们实验室于 1992 年使用具有不同转移潜力的大鼠乳腺腺癌细胞系和差异互补 DNA（cDNA）杂交方法，开始了一项识别候选转移相关基因的项目。在与低转移性细胞系相比，在高度转移性大鼠细胞系中大量过表达的基因中，我们于 1994 年发现了一个完全新颖的基因，该基因在数据库中没有任何同源或相关基因。该基因的全长 cDNA 被克隆、测序，并命名为 mta1（转移相关基因 1），最终，我们小组还克隆和测序了其人类 cDNA 对应物 MTA1。MTA1 现已被确定为基因家族（MTA 基因家族）的成员之一。MTA 基因的产物，MTA 蛋白，是转录共调节剂，在组蛋白脱乙酰化和核小体重塑中起作用。在这篇综述中，我们将简要讨论 mta1 基因及其人类对应物 MTA1 的鉴定和特性及其蛋白产物的研究。
