Perić Aleksandar, Baletić Nenad, Sotirović Jelena, Špadijer-Mirković Cveta
Department of Otorhinolaryngology, Faculty of Medicine, Military Medical Academy, Belgrade, Serbia
Department of Otorhinolaryngology, Faculty of Medicine, Military Medical Academy, Belgrade, Serbia.
Ann Otol Rhinol Laryngol. 2015 Apr;124(4):266-72. doi: 10.1177/0003489414554944. Epub 2014 Oct 14.
Eosinophil recruitment to the nasal mucosa involves a number of chemokines. The aim of this study was to evaluate nasal secretion levels of macrophage inflammatory protein-1 alpha (MIP-1α) and MIP-1β and to correlate these levels with clinical characteristics and degree of eosinophilia in nonallergic and allergic patients with nasal polyposis (NP).
Fourteen nonatopic and 14 atopic patients with NP were recruited for this cross-sectional study. Fourteen healthy subjects were included as controls. The concentrations of MIP-1α and MIP-1β in nasal secretions were measured by flow cytometry. Eosinophil counts were performed by cytological examination of the scraped nasal mucosa. We scored each of the 28 patients according to the nasal symptom score, endoscopic score, and computed tomography (CT) score.
We found significantly higher concentrations of MIP-1α in nasal fluid of nonallergic and allergic NP patients compared to control subjects. In nonallergic patients, we found positive correlations between MIP-1α levels and endoscopic score, CT score, and the percentage of eosinophils.
MIP-1α may play a role in eosinophil recruitment in NP. Our results suggest that the measurement of MIP-1α in nasal secretions could be useful in evaluating the degree of eosinophil inflammation and severity of disease in nonallergic patients.
嗜酸性粒细胞向鼻黏膜的募集涉及多种趋化因子。本研究旨在评估巨噬细胞炎性蛋白-1α（MIP-1α）和MIP-1β在鼻腔分泌物中的水平，并将这些水平与非变应性和变应性鼻息肉（NP）患者的临床特征及嗜酸性粒细胞增多程度相关联。
本横断面研究招募了14名非特应性和14名特应性NP患者。纳入14名健康受试者作为对照。通过流式细胞术测量鼻腔分泌物中MIP-1α和MIP-1β的浓度。通过刮取的鼻黏膜细胞学检查进行嗜酸性粒细胞计数。我们根据鼻症状评分、内镜评分和计算机断层扫描（CT）评分对28名患者中的每一位进行评分。
我们发现，与对照受试者相比，非变应性和变应性NP患者鼻腔液中MIP-1α的浓度显著更高。在非变应性患者中，我们发现MIP-1α水平与内镜评分、CT评分以及嗜酸性粒细胞百分比之间存在正相关。
MIP-1α可能在NP中嗜酸性粒细胞募集中起作用。我们的结果表明，测量鼻腔分泌物中的MIP-1α可能有助于评估非变应性患者中嗜酸性粒细胞炎症程度和疾病严重程度。
