Perić Aleksandar, Sotirović Jelena, Špadijer-Mirković Cveta, Matković-Jožin Svjetlana, Perić Aneta V, Vojvodić Danilo
Department of Otorhinolaryngology, Rhinology Unit, Faculty of Medicine, Military Medical Academy, Belgrade, Serbia.
Department of Otorhinolaryngology, Stavanger University Hospital, Stavanger, Norway.
Int Forum Allergy Rhinol. 2016 Apr;6(4):392-7. doi: 10.1002/alr.21684. Epub 2015 Dec 17.
An increased production of several chemoattractants, responsible for guiding the eosinophilic inflammatory process, has been reported in chronic rhinitis. The aim of this study was to evaluate nasal secretion levels of monocyte chemoattractant protein-1 (MCP-1), MCP-3, and regulated on activation normal T cell expressed and secreted (RANTES) and to correlate those levels with nasal symptoms and degree of eosinophilia in patients with nonallergic rhinitis with eosinophilia syndrome (NARES) and perennial allergic rhinitis (PAR).
Fourteen patients with PAR and 14 NARES patients were recruited for this cross-sectional study. Fourteen healthy subjects were included as controls. The concentrations of MCP-1, MCP-3, and RANTES in nasal secretions were measured using enzyme-linked immunosorbent assay (ELISA). Eosinophil counts were performed by percentage of differential granulocyte counts during cytological examination of scraped nasal mucosa obtained from the inferior turbinate. Therefore, we scored rhinitis patients according to nasal symptom score.
We found significantly higher concentrations of MCP-1 (p < 0.0001), MCP-3 (p = 0.018), and RANTES (p < 0.0001) in nasal fluid of NARES patients compared to patients with PAR. In PAR patients, we found positive correlation between RANTES levels and nasal symptom score and eosinophil counts. In patients with NARES, the concentrations of MCP-1 and RANTES were associated with nasal symptom score and percentage of eosinophils.
NARES is characterized by higher degree of eosinophilic inflammation than PAR. RANTES correlates well with the level of eosinophilic inflammation in both diseases. The measurement of chemokine levels in nasal secretions could be useful in evaluating the degree of chronic nasal inflammation.
据报道，慢性鼻炎中几种趋化因子的产生增加，这些趋化因子负责引导嗜酸性粒细胞炎症过程。本研究的目的是评估嗜酸性粒细胞增多综合征非过敏性鼻炎（NARES）和常年性变应性鼻炎（PAR）患者鼻分泌物中单核细胞趋化蛋白-1（MCP-1）、MCP-3和活化正常T细胞表达和分泌调节因子（RANTES）的水平，并将这些水平与鼻症状及嗜酸性粒细胞增多程度相关联。
本横断面研究招募了14例PAR患者和14例NARES患者。纳入14名健康受试者作为对照。采用酶联免疫吸附测定（ELISA）法测定鼻分泌物中MCP-1、MCP-3和RANTES的浓度。通过对从下鼻甲获取的刮取鼻黏膜进行细胞学检查时的粒细胞分类计数百分比来进行嗜酸性粒细胞计数。因此，我们根据鼻症状评分对鼻炎患者进行评分。
我们发现，与PAR患者相比，NARES患者鼻液中MCP-1（p < 0.0001）、MCP-3（p = 0.018）和RANTES（p < 0.0001）的浓度显著更高。在PAR患者中，我们发现RANTES水平与鼻症状评分及嗜酸性粒细胞计数之间呈正相关。在NARES患者中，MCP-1和RANTES的浓度与鼻症状评分及嗜酸性粒细胞百分比相关。
NARES的特征是嗜酸性粒细胞炎症程度高于PAR。RANTES在两种疾病中均与嗜酸性粒细胞炎症水平密切相关。测量鼻分泌物中趋化因子水平可能有助于评估慢性鼻炎症的程度。
