Santarelli A, Mascitti M, Orsini G, Memè L, Rocchetti R, Tiriduzzi P, Sampalmieri F, Putignano A, Procaccini M, Lo Muzio L, Bambini F
Department of Clinical, Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
Department of Pathologic Anatomy and Histopathology, Marche Polytechnic University, Ancona, Italy.
J Biol Regul Homeost Agents. 2014 Jul-Sep;28(3):523-9.
Calcium phosphate ceramics have been applied in bone replacement for several decades due to their excellent biocompatibility, bioactivity, osteo-conductivity and mechanical strength. Several studies have demonstrated that porous hydroxyapatite (HA) is an excellent scaffold for osteogenic proliferation and differentiation of the osteoprogenitor cells. However, different methods of synthesis and production of HA ceramic-based materials may have considerable effect on the physical and biological properties. In the present work, two hydroxyapatite-based materials, a natural hydroxyapatite ceramic of bovine origin and a synthetic nano-cristalline hydroxyapatite were tested in vitro with MG63 cell line. The results displayed that both the materials demonstrated a good biocompatibility. The immunocytochemical stain revealed a different positivity of the osteogenic markers between the cultures with the biomaterials, and the control culture. Western blot data confirmed the immunocytochemical stain. Both the materials tested in the present study demonstrated a good biocompatibility with the osteoblastic cells allowing, at the same time, the osteogenic differentiation, and they may be useful in clinical use.
磷酸钙陶瓷因其优异的生物相容性、生物活性、骨传导性和机械强度，已在骨替代领域应用了数十年。多项研究表明，多孔羟基磷灰石（HA）是骨祖细胞成骨增殖和分化的优良支架。然而，基于HA陶瓷材料的不同合成和生产方法可能会对其物理和生物学性质产生相当大的影响。在本研究中，用MG63细胞系对两种基于羟基磷灰石的材料进行了体外测试，一种是牛源天然羟基磷灰石陶瓷，另一种是合成纳米晶羟基磷灰石。结果显示，两种材料均表现出良好的生物相容性。免疫细胞化学染色显示，使用生物材料的培养物与对照培养物之间，成骨标志物的阳性情况不同。蛋白质印迹数据证实了免疫细胞化学染色结果。本研究中测试的两种材料均与成骨细胞表现出良好的生物相容性，同时允许成骨分化，它们可能在临床应用中有用。
