Mitrov D, Hadzi-Petrushev N, Stojkovski V, Gjorgievska E, Gagov H, Mladenov M
Faculty of Veterinary Medicine, Sts, Cyril and Methodius University, Skopje, Macedonia.
Faculty of Natural Sciences and Mathematics, Institute of Biology, Sts, Cyril and Methodius University, Skopje, Macedonia.
J Biol Regul Homeost Agents. 2014 Jul-Sep;28(3):531-5.
The aim of this study was to investigate the effect of chronic treatment with chromium hexavalent (Cr VI) on the platelet activation, inflammation and lipid peroxidation in rats. Thirty male Wistar rats weighing 251 ± 18 g were randomly assigned to one control and one Cr-exposed group. 8-iso-prostaglandin F(2α) (8-iso-PGF(2α)), interleukin 1β (IL-1β), tumor necrosis factor alpha (TNF-alpha) and creatinine (Crt), were measured in plasma, while 11-dehydro thromboxane B₂ (11-dehydro-TXB₂) in plasma andurine. Plasma levels of IL-1β, TNF-alpha, 8-iso-PGF(2α) and Crt were significantly increased in the Cr (VI)-treated in comparison to the control group. Also, in the urine of Cr (VI)-treated rats, 11-dehydro-TXB₂ was significantly increased in comparison to control rats. From the obtained data it is evident that chronic treatment with Cr (VI), accelerates arachidonic acid peroxidation in rats, which peroxidation further probably induces enhanced 11-dehydro-TXB₂ excretion rate.
本研究的目的是探讨六价铬（Cr VI）长期治疗对大鼠血小板活化、炎症和脂质过氧化的影响。将30只体重为251±18 g的雄性Wistar大鼠随机分为一个对照组和一个铬暴露组。检测血浆中的8-异前列腺素F（2α）（8-iso-PGF（2α））、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）和肌酐（Crt），同时检测血浆和尿液中的11-脱氢血栓素B₂（11-dehydro-TXB₂）。与对照组相比，Cr（VI）处理组的血浆IL-1β、TNF-α、8-iso-PGF（2α）和Crt水平显著升高。此外，与对照大鼠相比，Cr（VI）处理大鼠尿液中的11-脱氢-TXB₂显著增加。从获得的数据可以明显看出，Cr（VI）长期治疗会加速大鼠花生四烯酸过氧化，这种过氧化可能进一步导致11-脱氢-TXB₂排泄率增加。
