Department of Microsurgical Repair and Reconstruction, First Teaching Hospital of Xinjiang Medical University & Sports Medicine Research Center, Research Institute of Orthopedics of Xinjiang Uyghur Autonomous Region, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China.
Urumqi Center for Disease Control and Prevention, Urumqi 830026, Xinjiang Uyghur Autonomous Region, China.
Neural Regen Res. 2012 Dec 15;7(35):2801-10. doi: 10.3969/j.issn.1673-5374.2012.35.008.
Active Achilles tendon kinesitherapy facilitates the functional recovery of a ruptured Achilles tendon. However, protein expression during the healing process remains a controversial issue. New Zealand rabbits, aged 14 weeks, underwent tenotomy followed immediately by Achilles tendon microsurgery to repair the Achilles tendon rupture. The tendon was then immobilized or subjected to postoperative early motion treatment (kinesitherapy). Mass spectrography results showed that after 14 days of motion treatment, 18 protein spots were differentially expressed, among which, 12 were up-regulated, consisting of gelsolin isoform b and neurite growth-related protein collapsing response mediator protein 2. Western blot analysis showed that gelsolin isoform b was up-regulated at days 7-21 of motion treatment. These findings suggest that active Achilles tendon kinesitherapy promotes the neurite regeneration of a ruptured Achilles tendon and gelsolin isoform b can be used as a biomarker for Achilles tendon healing after kinesitherapy.
主动跟腱运动疗法促进跟腱断裂的功能恢复。然而，愈合过程中的蛋白质表达仍然是一个有争议的问题。14 周龄新西兰兔进行跟腱切断术，然后立即进行跟腱 microsurgery 修复跟腱断裂。然后将肌腱固定或进行术后早期运动治疗（运动疗法）。质谱结果显示，运动治疗 14 天后，有 18 个蛋白质斑点差异表达，其中 12 个上调，包括凝胶蛋白异构体 b 和神经突生长相关蛋白崩溃反应调节剂蛋白 2。Western blot 分析显示，运动治疗第 7-21 天凝胶蛋白异构体 b 上调。这些发现表明，主动跟腱运动疗法促进了断裂跟腱的神经突再生，凝胶蛋白异构体 b 可用作运动疗法后跟腱愈合的生物标志物。
