Qianman Bayixiati, Jialihasi Ayidaer, Asilehan Batiza, Kubai Aliya, Aibek Rakimbaiev, Wupuer Aikeremu, Tangkejie Wulanbai, Maimaitiaili Abudouheilil, Shawutali Nuerai, Badelhan Aynaz, Aizezi Adili, Aisaiding Amuding, Wuerliebieke Jianati, Bakyt Yerzat, Makemutibieke Elihaer, Jielile Jiasharete
Department of Orthopedics Centre, The First Teaching Hospital of Xinjiang Medical University, Kazakh Medical Association of Xinjiang Uygur Autonomous Region and The Sports Medicine Research Centre of Orthopedics Research Institute, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
Mol Med Rep. 2017 Sep;16(3):2355-2360. doi: 10.3892/mmr.2017.6889. Epub 2017 Jun 29.
Collapsin response mediator protein-2 (CRMP-2) is involved in neurite elongation and regeneration; however, its role in wound healing remains to be elucidated. The present study aimed to investigate the effects of active mobilization treatment on Achilles tendon healing and to determine the role of CRMP‑2 in the healing process. Sprague Dawley rats were subjected to Achilles tendon injury, which was verified by hematoxylin and eosin staining and scanning electronic microscopy. Immobilization induced the disruption of collagen fibril arrangement and promoted collagen fibril damage. The average collagen fibril perimeter in the active mobilization group was significantly increased compared with in the immobilization group (125.6±0.8 nm vs. 119.9±1.7 nm; P<0.05). In addition, immunohistological analysis revealed that CRMP‑2 expression was significantly upregulated, particularly in the ruptured site of Achilles tendon tissues derived from animals in the mobilization group compared with the immobilization group (0.32±0.00 vs. 0.08±0.00; P<0.05). The increased CRMP‑2 levels were also confirmed by western blotting (active mobilization group, 0.71±0.03; immobilization group, 0.49±0.01 nm; P<0.05). These results indicated that active mobilization may promote Achilles tendon healing via upregulation of CRMP‑2 protein expression.
塌陷反应介导蛋白2（CRMP-2）参与神经突的伸长和再生；然而，其在伤口愈合中的作用仍有待阐明。本研究旨在探讨主动活动治疗对跟腱愈合的影响，并确定CRMP-2在愈合过程中的作用。对Sprague Dawley大鼠进行跟腱损伤，通过苏木精-伊红染色和扫描电子显微镜进行验证。固定导致胶原纤维排列紊乱并促进胶原纤维损伤。主动活动组的平均胶原纤维周长与固定组相比显著增加（125.6±0.8nm对119.9±1.7nm；P<0.05）。此外，免疫组织学分析显示，与固定组相比，CRMP-2表达显著上调，尤其是在主动活动组动物的跟腱组织破裂部位（0.32±0.00对0.
