Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Neural Regen Res. 2014 Aug 1;9(15):1437-45. doi: 10.4103/1673-5374.139460.
A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside II inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5-2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit.
本研究旨在验证胡黄连苷 II 的神经保护作用，并优化其在脑缺血大鼠模型中的治疗时间窗和剂量。我们发现胡黄连苷 II 可抑制细胞凋亡，并降低脑缺血损伤后大鼠神经元特异性烯醇化酶（神经元损伤的标志物）的表达。根据甲苯胺蓝染色的结果，确定了最佳的治疗时间和剂量分别为：（1）2.0 小时和 10 mg/kg；（2）1.5 小时和 10 mg/kg；（3）2.0 小时和 10 mg/kg；（4）1.5 小时和 10 mg/kg。本研究结果表明，在脑缺血损伤后 1.5-2.0 小时腹腔内注射 10 mg/kg 胡黄连苷 II 对大鼠具有最佳的治疗效果。
