Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Neural Regen Res. 2014 Aug 1;9(15):1453-9. doi: 10.4103/1673-5374.139462.
Danhong injection (DHI), a Chinese Materia Medica standardized product extracted from Radix Salviae miltiorrhizae and Flos Carthami tinctorii, is widely used in China for treating acute ischemic stroke. In the present study, we explored the neuroprotective efficacy of DHI in a rat model of temporary middle cerebral artery occlusion, and evaluated the potential mechanisms underlying its effects. Pretreatment with DHI (0.9 and 1.8 mL/kg) resulted in a significantly smaller infarct volume and better neurological scores than pretreatment with saline. Furthermore, DHI significantly reduced the permeability of the blood-brain barrier, increased occludin protein expression and decreased neutrophil infiltration, as well as profoundly suppressing the upregulation of matrix metallopeptidase-9 expression seen in rats that had received vehicle. Matrix metallopeptidase-2 expression was not affected by ischemia or DHI. Moreover, DHI (1.8 mL/kg) administered 3 hours after the onset of ischemia also improved neurological scores and reduced infarct size. Our results indicate that the neuroprotective efficacy of DHI in a rat model of cerebral ischemia-reperfusion injury is mediated by a protective effect on the blood-brain barrier and the reversal of neutrophil infiltration.
丹红注射液（DHI）是一种从丹参和红花中提取的中药标准化产品，在中国广泛用于治疗急性缺血性脑卒中。在本研究中，我们探讨了 DHI 在大鼠短暂性大脑中动脉闭塞模型中的神经保护作用，并评估了其作用的潜在机制。与生理盐水预处理相比，DHI（0.9 和 1.8 mL/kg）预处理可显著减小梗死体积和改善神经功能评分。此外，DHI 可显著降低血脑屏障通透性，增加紧密连接蛋白表达，减少中性粒细胞浸润，并显著抑制缺血大鼠基质金属蛋白酶-9 表达的上调。基质金属蛋白酶-2 的表达不受缺血或 DHI 的影响。此外，在缺血后 3 小时给予 DHI（1.8 mL/kg）也可改善神经功能评分并减小梗死体积。我们的结果表明，DHI 在大鼠脑缺血再灌注损伤模型中的神经保护作用是通过对血脑屏障的保护作用和逆转中性粒细胞浸润来介导的。
