Zandkarimi Majid, Shafaati Alireza, Foroutan Sayyed Mohsen, A Lucy Charles
School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran.
School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2012 Winter;11(1):129-36.
In chiral and non-chiral electrophoretic resolution of basic drugs, adsorption of analytes to negatively charged capillary wall could lead to poor repeatability of migration time and peak area. In addition, chiral resolutions of basic drugs are commonly performed in low pH buffers. Therefore, longer analysis time due to suppression of electroosmotic flow (EOF) is another dilemma. In this work the improvement effect of polybrene (PB), a cationic polymer, on chiral separation of a model basic drug, amlodipine (AML), was investigated. PB both as a semi-permanent coating agent and as an additive in the running buffer was utilized. Better results were obtained with PB as a buffer additive. Compare to untreated bare silica without using PB in running buffer, addition of 0.0005% PB buffer decreased analysis time downed to 3 folds; efficiency improved up to 5 folds; limit of detection (LOD) and limit of quantification (LOQ) downed to 8 folds and within-day migration time and peak area repeatabilities, in terms of relative standard deviations (RSD) downed to 5 and 20 folds, respectively.
在碱性药物的手性和非手性电泳分离中，分析物吸附到带负电荷的毛细管壁上可能导致迁移时间和峰面积的重复性较差。此外，碱性药物的手性分离通常在低pH缓冲液中进行。因此，由于电渗流（EOF）受到抑制而导致分析时间延长是另一个难题。在这项工作中，研究了阳离子聚合物聚乙烯亚胺（PB）对模型碱性药物氨氯地平（AML）手性分离的改善效果。PB既用作半永久性涂层剂，也用作运行缓冲液中的添加剂。以PB作为缓冲添加剂可获得更好的结果。与在运行缓冲液中不使用PB的未处理裸硅胶相比，添加0.0005% PB缓冲液可将分析时间缩短至三分之一；效率提高至五倍；检测限（LOD）和定量限（LOQ）降低至八分之一，日内迁移时间和峰面积重复性，以相对标准偏差（RSD）计分别降低至五分之一和二十分之一。
