Silva Maria Luiza, Melo Victor Hugo, Aleixo Agdemir Waléria, Aleixo Lúcia Fernandes, Pascoal-Xavier Marcelo Antônio, Silva Rafaela Oliveira, Ferreira Laís Alves, Domingos Willian Cunha, Greco Dirceu Bartolomeu
Laboratório Central, Hospital das Clínicas, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Mem Inst Oswaldo Cruz. 2014 Sep;109(6):775-81. doi: 10.1590/0074-0276140140. Epub 2014 Sep 9.
Understanding the social conditions and immunological characteristics that allow some human immunodeficiency virus (HIV)-exposed patients to remain uninfected represents an on-going challenge. In this study, the socio-demographic and sexual behaviour characteristics and immune activation profiles of uninfected individuals exposed to HIV-infected partners were investigated. A confidential and detailed questionnaire was administered and venous blood was tested using HIV-1/enzyme immunoassays, plasma HIV-1 RNA levels/bDNA and immunophenotyping/flow cytometry to determine the frequencies of CD4 and CD8 T cells expressing activation markers. The data analysis showed significant differences (p < 0.05) for immune parameters in individuals who were uninfected, albeit exposed to HIV-infected partners, compared with unexposed individuals. In particular, the exposed, uninfected individuals had a higher frequency (median, minimum-maximum) of CD4⁺HLA-DR⁺ (4.2, 1.8-6.1), CD8⁺HLA-DR⁺ (4.6, 0.9-13.7), CD4⁺CD45RO⁺ (27.5, 14.2-46.6), CD4⁺CD45RO⁺CD62L⁺ (46.7, 33.9-67.1), CD8⁺CD45RA⁺HLA-DR⁺ (12.1, 3.4-35.8) and CD8⁺CD45RO⁺HLA-DR⁺ (9.0, 3.2-14.8) cells, a decreased percentage of CD8⁺CD28⁺ cells (11.7, 4.5-24.0) and a lower cell-surface expression of Fcγ-R/CD16 on monocytes (56.5, 22.0-130.0). The plasma HIV-1 RNA levels demonstrated detectable RNA virus loads in 57% of the HIV-1⁺ female partners. These findings demonstrate an activation profile in both CD4 and CD8 peripheral T cells from HIV-1 exposed seronegative individuals of serodiscordant couples from a referral centre in Belo Horizonte, state of Minas Gerais.
了解使一些暴露于人类免疫缺陷病毒（HIV）的患者仍未感染的社会状况和免疫特征是一项持续的挑战。在本研究中，对暴露于HIV感染伴侣的未感染个体的社会人口统计学和性行为特征以及免疫激活情况进行了调查。采用一份保密且详细的问卷，并使用HIV-1酶免疫测定、血浆HIV-1 RNA水平分支DNA检测以及免疫表型分析/流式细胞术检测静脉血，以确定表达激活标志物的CD4和CD8 T细胞的频率。数据分析显示，与未暴露个体相比，尽管暴露于HIV感染伴侣但仍未感染的个体在免疫参数方面存在显著差异（p < 0.05）。特别是，暴露的未感染个体中，CD4⁺HLA-DR⁺（中位数，最小值 - 最大值：4.2，1.8 - 6.1）、CD8⁺HLA-DR⁺（4.6，0.9 - 13.7）、CD4⁺CD45RO⁺（27.5，14.2 - 46.6）、CD4⁺CD45RO⁺CD62L⁺（46.7，33.9 - 67.1）、CD8⁺CD45RA⁺HLA-DR⁺（12.1，3.4 - 35.8）和CD8⁺CD45RO⁺HLA-DR⁺（9.0，3.2 - 14.8）细胞的频率较高，CD8⁺CD28⁺细胞的百分比降低（11.7，4.5 - 24.0），单核细胞上Fcγ-R/CD16的细胞表面表达较低（56.5，22.0 - 130.0）。血浆HIV-1 RNA水平显示，57%的HIV-1⁺女性伴侣中可检测到RNA病毒载量。这些发现表明，来自米纳斯吉拉斯州贝洛奥里藏特一个转诊中心的血清学不一致夫妇中，HIV-1暴露血清阴性个体的CD4和CD8外周T细胞均呈现激活状态。
