Grzegorek Irmina, Drozdz Katarzyna, Chmielewska Magdalena, Gomulkiewicz Agnieszka, Jablonska Karolina, Piotrowska Aleksandra, Karczewski Maciej, Janczak Dariusz, Podhorska-Okolow Marzena, Dziegiel Piotr, Szuba Andrzej
1 Regional Specialized Hospital in Wroclaw, Research and Development Center , Wroclaw, Poland .
Lymphat Res Biol. 2014 Dec;12(4):222-31. doi: 10.1089/lrb.2013.0048.
Twenty-six iliac artery segments were divided in two groups: atherosclerotic (A) and nonatherosclerotic (NA). Expression of LYVE-1, VEGF-C, VEGF-D, and CCR7 receptor were studied with immunohistochemistry (IHC) and Western blot (WB). IHC was performed on 26 samples of iliac arteries obtained from deceased 19 organ donors. The samples were divided into an atherosclerotic group (A) [subjects with history of cardiovascular disease (hypertension, ischemic heart disease) or/and diabetes] (n=16), and a nonatherosclerotic group (NA) [subjects without any known cardiovascular diseases or cardiovascular risk factors] (n=10). WB was performed on 19 iliac artery segments obtained from two groups, based on clinical data: an atherosclerotic group (A) [patients with atherosclerosis, who underwent surgery for lower limb ischemia] (n=10), and a nonatherosclerotic group (NA) [deceased organ donors without cardiovascular diseases/risk factors (n=9)]. Expression of LYVE-1, VEGF-C, VEGF-D, and CCR-7 was increased in atherosclerotic arteries. Positive correlations between LYVE-1 and VEGF-C expression in the intima-media complex assessed by IHC: (r=0.54; p=0.005) and WB: (r=0.47; p=0.005) were found. Positive correlations between expression of CCR-7 and other markers were observed. Lymphangiogenesis is enhanced within the atherosclerotic arterial wall. Our results confirm lymphatic system activation with increased lymphangiogenesis and lymphocyte/macrophage trafficking in atherosclerosis.
26个髂动脉节段被分为两组：动脉粥样硬化组（A组）和非动脉粥样硬化组（NA组）。采用免疫组织化学（IHC）和蛋白质印迹法（WB）研究淋巴管内皮透明质酸受体1（LYVE-1）、血管内皮生长因子C（VEGF-C）、血管内皮生长因子D（VEGF-D）和CC趋化因子受体7（CCR7）的表达。对从19名已故器官捐献者获取的26个髂动脉样本进行IHC检测。样本被分为动脉粥样硬化组（A组）[有心血管疾病（高血压、缺血性心脏病）病史或/和糖尿病的受试者]（n = 16）和非动脉粥样硬化组（NA组）[无任何已知心血管疾病或心血管危险因素的受试者]（n = 10）。根据临床资料，对从两组获取的19个髂动脉节段进行WB检测：动脉粥样硬化组（A组）[因下肢缺血接受手术的动脉粥样硬化患者]（n = 10）和非动脉粥样硬化组（NA组）[无心血管疾病/危险因素的已故器官捐献者]（n = 9）。动脉粥样硬化动脉中LYVE-1、VEGF-C、VEGF-D和CCR-7的表达增加。通过IHC评估发现，在内膜-中膜复合体中LYVE-1与VEGF-C表达之间存在正相关：（r = 0.54；p = 0.005），通过WB评估也存在正相关：（r = 0.47；p = 0.005）。观察到CCR-7与其他标志物表达之间存在正相关。动脉粥样硬化动脉壁内的淋巴管生成增强。我们的结果证实，在动脉粥样硬化中，淋巴系统被激活，淋巴管生成增加，淋巴细胞/巨噬细胞运输增多。
