Rajabi Khadijeh
Department of Chemistry, University of British Columbia (UBC), 2036 Mail Mall, Vancouver, BC, V6T 1Z1, Canada,
J Am Soc Mass Spectrom. 2015 Jan;26(1):71-82. doi: 10.1007/s13361-014-1004-y. Epub 2014 Oct 16.
A pulsed hydrogen/deuterium exchange (HDX) method has been developed for rapid monitoring of the exchange kinetics of protein ions with D2O a few milliseconds after electrospray ionization (ESI). The stepwise gradual evolution of HDX of multiply charged protein ions was monitored using the pulsed HDX mass spectrometry technique. Upon introducing a very short pulse of D2O (in the μs to ms time scale) into the linear ion trap (LIT) of a time-of-flight (TOF) mass spectrometer, bimodal distributions were detected for the ions of cytochrome c and ubiquitin. Mechanistic details of HDX reactions for ubiquitin and cytochrome c in the gas phase were uncovered and the structural transitions were followed by analyzing the kinetics of HDX.
已开发出一种脉冲氢/氘交换（HDX）方法，用于在电喷雾电离（ESI）后几毫秒内快速监测蛋白质离子与D2O的交换动力学。使用脉冲HDX质谱技术监测多电荷蛋白质离子HDX的逐步演变。在将非常短的D2O脉冲（在微秒至毫秒时间尺度内）引入飞行时间（TOF）质谱仪的线性离子阱（LIT）中后，检测到细胞色素c和泛素离子的双峰分布。揭示了气相中泛素和细胞色素c的HDX反应的机理细节，并通过分析HDX动力学跟踪结构转变。
