Bhattarai Sushila, Alany Raid G, Bunt Craig R, Abdelkader Hamdy, Rathbone Michael J
Faculty of Medical and Health Sciences, School of Pharmacy, The University of Auckland, Auckland, New Zealand.
AAPS J. 2015 Jan;17(1):167-74. doi: 10.1208/s12248-014-9678-4. Epub 2014 Oct 16.
This manuscript reports (for the first time) on antibiotic-free polymeric inserts for the prevention and/or treatment of bovine mastitis. Polyethylene oxide (PEO)-based inserts were prepared using different concentrations of various hydrophilic polymers and water-soluble and water-insoluble drug-release-modifying excipients. A simple and scalable melt-extrusion method was employed to prepare the inserts. The prepared inserts were characterised for their dimension, rheological and mechanical properties. The in vitro release of a model bacteriostatic drug (salicylic acid) from the prepared inserts was studied to demonstrate the effectiveness and reproducibility of the melt-extrusion manufacturing method. Further, the in vitro stability of the inserts was evaluated using gel permeation chromatography (GPC) to monitor any change in molecular weight under real-time and accelerated storage conditions. The investigated inserts were stable at accelerated storage conditions over a period of 6 months. PEO inserts have the potential to serve a dual purpose, act as a physical barrier against pathogens invading the teat canal of cows and possibly control the release of a drug.
本手稿首次报道了用于预防和/或治疗牛乳腺炎的无抗生素聚合物插入物。使用不同浓度的各种亲水性聚合物以及水溶性和水不溶性药物释放改性辅料制备了基于聚环氧乙烷（PEO）的插入物。采用一种简单且可扩展的熔融挤出方法制备插入物。对制备的插入物的尺寸、流变学和机械性能进行了表征。研究了模型抑菌药物（水杨酸）从制备的插入物中的体外释放情况，以证明熔融挤出制造方法的有效性和可重复性。此外，使用凝胶渗透色谱法（GPC）评估了插入物的体外稳定性，以监测在实时和加速储存条件下分子量的任何变化。所研究的插入物在加速储存条件下6个月内保持稳定。PEO插入物有潜力发挥双重作用，作为抵御病原体侵入奶牛乳头管的物理屏障，并可能控制药物的释放。
