Joh Hea Min, Choi Ji Ye, Kim Sun Ja, Chung T H, Kang Tae-Hong
Department of Physics, Dong-A University, Busan 604-714, Republic of Korea.
Department of Biological Science, Dong-A University, Busan 604-714, Republic of Korea.
Sci Rep. 2014 Oct 16;4:6638. doi: 10.1038/srep06638.
The atmospheric pressure helium plasma jet driven by pulsed dc voltage was utilized to treat human lung cancer cells in vitro. The properties of plasma plume were adjusted by the injection type and flow rate of additive oxygen gas in atmospheric pressure helium plasma jet. The plasma characteristics such as plume length, electric current and optical emission spectra (OES) were measured at different flow rates of additive oxygen to helium. The plasma plume length and total current decreased with an increase in the additive oxygen flow rate. The electron excitation temperature estimated by the Boltzmann plot from several excited helium emission lines increased slightly with the additive oxygen flow. The oxygen atom density in the gas phase estimated by actinometry utilizing argon was observed to increase with the additive oxygen flow. The concentration of intracellular reactive oxygen species (ROS) measured by fluorescence assay was found to be not exactly proportional to that of extracellular ROS (measured by OES), but both correlated considerably. It was also observed that the expression levels of p53 and the phospho-p53 were enhanced in the presence of additive oxygen flow compared with those from the pure helium plasma treatment.
利用脉冲直流电压驱动的大气压氦等离子体射流在体外处理人肺癌细胞。通过在大气压氦等离子体射流中添加氧气的注入类型和流速来调节等离子体羽流的特性。在不同的氦气与添加氧气的流速下，测量了等离子体羽流长度、电流和发射光谱（OES）等等离子体特性。随着添加氧气流速的增加，等离子体羽流长度和总电流减小。通过对几条激发态氦发射线的玻尔兹曼图估算的电子激发温度随添加氧气流量略有增加。利用氩气通过光化学测定法测得的气相中氧原子密度随添加氧气流量增加。通过荧光测定法测得的细胞内活性氧（ROS）浓度与细胞外ROS（通过OES测量）浓度并非完全成正比，但两者相关性显著。还观察到，与纯氦等离子体处理相比，在有添加氧气流量的情况下，p53和磷酸化p53的表达水平有所提高。
