Thomas Giju, van Voskuilen Johan, Truong Hoa, Gerritsen Hans C, Sterenborg H J C M
Department of Biomedical Engineering and Physics, Academic Medical Centre, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
Centre for Optical Diagnostics and Therapy, Erasmus Medical Centre, Post Box 2040, 3000 CA, Rotterdam, The Netherlands.
J Biophotonics. 2015 Aug;8(8):668-80. doi: 10.1002/jbio.201400074. Epub 2014 Oct 15.
Early detection of cutaneous squamous cell carcinoma (cSCC) can enable timely therapeutic and preventive interventions for patients. In this study, in vivo nonlinear optical imaging (NLOI) based on two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG), was used to non-invasively detect microscopic changes occurring in murine skin treated topically with 7,12-dimethylbenz(a)anthracene (DMBA). The optical microscopic findings and the measured TPEF-SHG index show that NLOI was able to clearly detect early cytostructural changes in DMBA treated skin that appeared clinically normal. This suggests that in vivo NLOI could be a non-invasive tool to monitor early signs of cSCC. In vivo axial NLOI scans of normal murine skin (upper left), murine skin with preclinical hyperplasia (upper right), early clinical murine skin lesion (lower left) and late or advanced murine skin lesion (lower right).
皮肤鳞状细胞癌（cSCC）的早期检测能够为患者及时开展治疗和预防干预措施。在本研究中，基于双光子激发荧光（TPEF）和二次谐波产生（SHG）的体内非线性光学成像（NLOI）被用于非侵入性检测局部涂抹7,12-二甲基苯并(a)蒽（DMBA）的小鼠皮肤中发生的微观变化。光学显微镜检查结果和所测得的TPEF-SHG指数表明，NLOI能够清晰检测出经DMBA处理后临床上看似正常的皮肤中的早期细胞结构变化。这表明体内NLOI可能是一种监测cSCC早期迹象的非侵入性工具。正常小鼠皮肤（左上）、具有临床前增生的小鼠皮肤（右上）、早期临床小鼠皮肤病变（左下）和晚期或进展期小鼠皮肤病变（右下）的体内轴向NLOI扫描图。
