Kim Jaeyoung, Lee Onseok, Ha Seunghan, Lee Jung Woo, Oh Chilhwan
Research Institute for Skin Image, College of Medicine, Korea University, Seoul, South Korea.
Department of Medical IT Engineering, College of Medical Sciences, Soonchunhyang University, Chungnam, South Korea.
J Fluoresc. 2017 Jan;27(1):13-20. doi: 10.1007/s10895-016-1931-z. Epub 2016 Sep 16.
Early diagnosis is one of the most important factors that increase the therapeutic potential of the disease. Diagnoses conducted by conventional equipment are expensive, time-consuming, burdensome to patients, and do not have high success rates. Diagnostic methods have also been investigated using nanoparticles. However, there have been no significant improvements in the early diagnosis of disease. The diagnosis technique proposed in this paper consumes less time, is more cost-effective, and more accurate. It uses a new concept-a low-intensity fluorescence molecular imaging system with a lock-in technique. This study applied the lock-in technique to basic research in contrast enhancement and optimization. This improved fluorescence distribution analysis, resulting in increased resolution of optical molecular imaging for early diagnosis of disease. An experimental lock-in fluorescence imaging system, which used a variety of fluorescent dyes, achieved signal amplification 100 times greater than that of a conventional fluorescence imaging system. The results of this study demonstrate that the lock-in technique could significantly improve optical molecular imaging technology, making it possible to achieve early diagnosis of disease.
早期诊断是提高疾病治疗潜力的最重要因素之一。使用传统设备进行诊断成本高昂、耗时、给患者带来负担，且成功率不高。也有人研究了使用纳米颗粒的诊断方法。然而，疾病的早期诊断并没有显著改善。本文提出的诊断技术耗时更少、更具成本效益且更准确。它采用了一种新概念——具有锁相技术的低强度荧光分子成像系统。本研究将锁相技术应用于对比度增强和优化的基础研究。这改进了荧光分布分析，提高了用于疾病早期诊断的光学分子成像分辨率。一个使用多种荧光染料的实验性锁相荧光成像系统实现了比传统荧光成像系统大100倍的信号放大。本研究结果表明，锁相技术可以显著改进光学分子成像技术，从而实现疾病的早期诊断。
