Nakashima Yuko, Abe Naoko, Ito Yoshihiro, Abe Hiroshi
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Methods Mol Biol. 2015;1218:17-36. doi: 10.1007/978-1-4939-1538-5_2.
We synthesized three types of nanostructured RNAs that induce RNA interference (RNAi): branched RNAs, dumbbell-shaped RNA, and circular double-stranded RNAs. All three nanostructured RNAs were transformed into double-stranded RNA of approximately 20 base pairs when they were treated with nuclease enzymes such as Dicer. These dsRNA species induced gene silencing when they are were introduced into mammalian cells.
我们合成了三种可诱导RNA干扰（RNAi）的纳米结构RNA：分支RNA、哑铃状RNA和环状双链RNA。当用诸如Dicer等核酸酶处理时，所有这三种纳米结构RNA均转化为约20个碱基对的双链RNA。当将这些双链RNA种类引入哺乳动物细胞时，它们会诱导基因沉默。
