基于实时聚合酶链式反应从血样中鉴定细菌和真菌病原体

Real-time PCR-based identification of bacterial and fungal pathogens from blood samples.

作者信息

Mai Madeleine, Müller Iris, Maneg Daniela, Lohr Benedikt, Haecker Achim, Haberhausen Gerd, Hunfeld Klaus-Peter

机构信息

Institute of Laboratory Medicine, Microbiology and Infection Control, Northwest Medical Centre, Frankfurt/Main, Germany.

出版信息

Methods Mol Biol. 2015;1237:139-47. doi: 10.1007/978-1-4939-1776-1_14.

DOI:10.1007/978-1-4939-1776-1_14

PMID:25319787

Abstract

Latest major contributions in the field of sepsis diagnostics result from advances in PCR technologies permitting new standards in speed and quality, given the fact that a timely diagnosis is the decisive factor to the survival of patients with bloodstream infections.Multiplex real-time PCR is a quantitative method for simultaneous amplification and detection of different targeted DNA molecules within hours. Nevertheless, various studies have shown a number of technical shortcomings as well as a high heterogeneity in sensitivity.The present method allows the standardized and rapid detection and identification of 25 common bacteria and fungi responsible for bloodstream infections from whole blood samples by using LightCycler(®) SeptiFast (LC-SF) test, based on real-time PCR.

摘要

脓毒症诊断领域的最新主要贡献来自于聚合酶链反应（PCR）技术的进步，鉴于及时诊断是血流感染患者生存的决定性因素，这些技术在速度和质量方面达到了新的标准。多重实时PCR是一种在数小时内同时扩增和检测不同目标DNA分子的定量方法。然而，各种研究已经表明该方法存在一些技术缺陷以及灵敏度方面的高度异质性。目前的方法通过使用基于实时PCR的LightCycler(®) SeptiFast（LC-SF）检测，能够从全血样本中对导致血流感染的25种常见细菌和真菌进行标准化快速检测和鉴定。