Sakha Agricultural Research Station, Field Crop Research Institute, Agricultural Research Center , P.O. Box 33717, Kafr Sl-Sheikh , Egypt.
Kazusa DNA Research Institute , 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818 , Japan.
Breed Sci. 2014 Sep;64(3):252-63. doi: 10.1270/jsbbs.64.252. Epub 2014 Sep 1.
To develop a high density linkage map in faba bean, a total of 1,363 FBES (Faba bean expressed sequence tag [EST]-derived simple sequence repeat [SSR]) markers were designed based on 5,090 non-redundant ESTs developed in this study. A total of 109 plants of a 'Nubaria 2' × 'Misr 3' F2 mapping population were used for map construction. Because the parents were not pure homozygous lines, the 109 F2 plants were divided into three subpopulations according to the original F1 plants. Linkage groups (LGs) generated in each subpopulation were integrated by commonly mapped markers. The integrated 'Nubaria 2' × 'Misr 3' map consisted of six LGs, representing a total length of 684.7 cM, with 552 loci. Of the mapped loci, 47% were generated from multi-loci diagnostic (MLD) markers. Alignment of homologous sequence pairs along each linkage group revealed obvious syntenic relationships between LGs in faba bean and the genomes of two model legumes, Lotus japonicus and Medicago truncatula. In a polymorphic analysis with ten Egyptian faba bean varieties, 78.9% (384/487) of the FBES markers showed polymorphisms. Along with the EST-SSR markers, the dense map developed in this study is expected to accelerate marker assisted breeding in faba bean.
为了在蚕豆中开发高密度连锁图谱，本研究共设计了 1363 个 FBES（蚕豆表达序列标签[EST]-衍生简单序列重复[SSR]）标记，基于 5090 个非冗余 EST。使用'Nubaria 2'×'Misr 3'F2 作图群体中的 109 株植物进行图谱构建。由于亲本不是纯合系，109 株 F2 植物根据原始 F1 植物分为三个亚群。通过共同映射的标记整合每个亚群中生成的连锁群（LG）。整合的'Nubaria 2'×'Misr 3'图谱由六个 LG 组成，总长度为 684.7cM，有 552 个位点。在映射的位点中，47%是由多位点诊断（MLD）标记产生的。沿着每个连锁群的同源序列对的比对揭示了蚕豆中的 LG 与两种模式豆科植物，绿豆和紫花苜蓿之间存在明显的同线性关系。在对十个埃及蚕豆品种进行的多态性分析中，78.9%（384/487）的 FBES 标记表现出多态性。结合 EST-SSR 标记，本研究中开发的高密度图谱有望加速蚕豆的标记辅助育种。
