Peest D, de Vries I, Hölscher R, Leo R, Deicher H
Abteilung Immunologie und Transfusionsmedizin, Medizinische Hochschule Hannover, Federal Republic of Germany.
Cancer Immunol Immunother. 1989;30(4):227-32. doi: 10.1007/BF01665009.
Short-term cultures containing bone marrow mononuclear cells from multiple myeloma patients secrete monoclonal immunoglobulin- and beta 2-microglobulin into the supernatant, which can be measured quantitatively in an enzyme-linked immunosorbant assay. In this system, the addition of interleukin-2 was shown to induce tumor cell regression in the cultures from 10 out of 14 multiple myeloma patients in a dose-dependent manner. Marker analyses of culture cell populations indicate that OKT3 antibody or interleukin-2 did not directly act on the malignant clone but augmented autologous T lymphocytes, which were responsible for the regression of tumor cells in the cultures.
含有多发性骨髓瘤患者骨髓单个核细胞的短期培养物会将单克隆免疫球蛋白和β2-微球蛋白分泌到上清液中，可通过酶联免疫吸附测定法定量检测。在该系统中，已表明添加白细胞介素-2能以剂量依赖方式诱导14例多发性骨髓瘤患者中10例患者培养物中的肿瘤细胞消退。培养细胞群体的标志物分析表明，OKT3抗体或白细胞介素-2并非直接作用于恶性克隆，而是增强了自体T淋巴细胞，后者导致了培养物中肿瘤细胞的消退。
