Lee Hyun-Ah, Song Yeong-Ok, Jang Mi-Soon, Han Ji-Sook
Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Korea.
Food and Safety Research Center, National Fisheries Research & Development Institute, Busan 619-705, Korea.
Prev Nutr Food Sci. 2014 Sep;19(3):170-7. doi: 10.3746/pnf.2014.19.3.170.
Endothelial cell dysfunction is considered to be a major cause of vascular complications in diabetes. In the present study, we investigated the protective effect of a baechu kimchi added Ecklonia cava extract (BKE) against high glucose induced oxidative damage in human umbilical vein endothelial cells (HUVECs). Treatment with a high concentration of glucose (30 mM) induced cytotoxicity, whereas treatment with BKE protected HUVECs from high glucose induced damage; by restoring cell viability. In addition, BKE reduced lipid peroxidation, intracellular reactive oxygen species and nitric oxide levels in a dose dependent manner. Treatment with high glucose concentrations also induced the overexpression of inducible nitric oxide synthase, cyclooxygenase-2 and NF-κB proteins in HUVECs, but BKE treatment significantly reduced the overexpression of these proteins. These findings indicate that BKE may be a valuable treatment against high glucose-induced oxidative stress HUVECs.
内皮细胞功能障碍被认为是糖尿病血管并发症的主要原因。在本研究中，我们调查了添加了海蕴提取物的白菜泡菜（BKE）对高糖诱导的人脐静脉内皮细胞（HUVECs）氧化损伤的保护作用。高浓度葡萄糖（30 mM）处理诱导细胞毒性，而BKE处理可保护HUVECs免受高糖诱导的损伤，通过恢复细胞活力实现。此外，BKE以剂量依赖性方式降低脂质过氧化、细胞内活性氧和一氧化氮水平。高葡萄糖浓度处理还诱导HUVECs中诱导型一氧化氮合酶、环氧化酶-2和NF-κB蛋白的过表达，但BKE处理显著降低了这些蛋白的过表达。这些发现表明，BKE可能是一种对抗高糖诱导的HUVECs氧化应激的有效治疗方法。
