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数字全息显微镜中利用虚拟共焦方案补偿相位像差

Compensation of phase aberration by using a virtual confocal scheme in digital holographic microscopy.

作者信息

Chew Yang-Kun, Shiu Min-Tzung, Wang Je-Chung, Chang Chi-Ching

出版信息

Appl Opt. 2014 Sep 20;53(27):G184-91. doi: 10.1364/AO.53.00G184.

DOI:10.1364/AO.53.00G184
PMID:25322128
Abstract

This work presents cost-effective, simple arbitrary phase-step digital holographic microscopy to suppress both zero-order and twin-image terms. A virtual confocal offset lens under in-line configuration is also used to compensate for the introduced quadratic phase by using a microscope objective lens. In addition to reducing the difficulties of physical confocal configurations, the proposed method significantly increases the magnification power, ultimately achieving the purposes of an optical zoom. An attempt is also made to reduce the noise interference of a high magnification system by developing a long focal lens to reduce light detection size, subsequently gaining an approximately plane wave light source to illuminate the object within the effective depth of focus. Experimental results indicate that the proposed high magnification system can be elevated with low noise interference, and image reconstruction without quadratic phase terms.

摘要

这项工作提出了一种经济高效、简单的任意相位步进数字全息显微镜技术,以抑制零级项和孪生像项。在线配置下的虚拟共焦偏移透镜也被用于通过使用显微镜物镜来补偿引入的二次相位。除了减少物理共焦配置的困难之外,所提出的方法还显著提高了放大倍数,最终实现了光学变焦的目的。还尝试通过开发长焦透镜来减小光检测尺寸,从而减少高放大倍数系统的噪声干扰,随后获得近似平面波光源以在有效焦深内照亮物体。实验结果表明,所提出的高放大倍数系统可以在低噪声干扰下提升,并且能够进行无二次相位项的图像重建。

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