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在浮游生物研究中使用透镜改进数字加博尔全息显微镜

Improvement of digital Gabor holographic microscopy using a lens in plankton studies.

作者信息

Arias-Sosa Yaumel C, Moreno-Vega Gelaysi, Lopes Rubens M, Valin-Rivera José-Luis, Valin-Fernández Meylí, Gonçalves Edison, Ricardo-Pérez Jorge O

机构信息

Physics Department, Faculty of Natural and Exact Sciences, Universidad de Oriente, Santiago de Cuba, Cuba.

Physics Department, Higher Institute for Mining-Metallurgical, Moa, Holguín, Cuba.

出版信息

Heliyon. 2024 Apr 21;10(9):e29441. doi: 10.1016/j.heliyon.2024.e29441. eCollection 2024 May 15.

DOI:10.1016/j.heliyon.2024.e29441
PMID:38694032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11058717/
Abstract

This work utilizes a Gabor Holographic Optical Scheme integrated with a microscope objective and a thin convex plane lens. This bi-telecentric lens system corrects spherical aberration from the objective, maintains consistent magnification across various reconstruction distances, and ensures a plane incidence on CMOS. Depending on the focal lengths of the objective and lens, the final image can be enlarged or reduced compared to the classic Gabor system, resulting in high-quality reconstructed phase images without spherical aberration. This setup was employed to capture phase distribution and intensity images of planktonic objects, such as copepods, achieving superior image quality.

摘要

这项工作采用了一种与显微镜物镜和薄凸平透镜集成的加博尔全息光学方案。这种双远心透镜系统校正了物镜的球差，在不同的重建距离上保持一致的放大率，并确保在CMOS上的平面入射。根据物镜和透镜的焦距，与经典加博尔系统相比，最终图像可以放大或缩小，从而得到无球差的高质量重建相位图像。该装置用于捕获浮游生物（如桡足类）的相位分布和强度图像，获得了卓越的图像质量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/b36224448702/gr009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/5d5a45452399/gr001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/e8fb2fd60e61/gr002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/098d20847dee/gr003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/b96cfda38068/gr004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/5330f522872a/gr005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/769e4a067da2/gr006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/14092c863d9c/gr007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/d881f371b0f6/gr008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/b36224448702/gr009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/5d5a45452399/gr001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/e8fb2fd60e61/gr002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/098d20847dee/gr003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/b96cfda38068/gr004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/5330f522872a/gr005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/769e4a067da2/gr006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/14092c863d9c/gr007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/d881f371b0f6/gr008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce8d/11058717/b36224448702/gr009.jpg

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