Morita E, Schröder J M, Christophers E
J Dermatol. 1989 Oct;16(5):348-51. doi: 10.1111/j.1346-8138.1989.tb01278.x.
We determined the chemotactic responsiveness of peripheral eosinophilic granulocytes (eosinophils) isolated from patients with inflammatory dermatoses and healthy volunteers. Ten patients with atopic dermatitis, five patients with drug reactions, ten patients with psoriasis, and fourteen healthy volunteers were studied. Well characterized chemotaxins, the complement split product C5a, leukotriene B4 (LTB4), platelet activating factor (PAF), and N-formyl-methionyl-leucyl-phenylalanine (FMLP), were used as chemoattractants. Eosinophils from healthy volunteers showed strong migratory responses towards C5a and PAF but responded poorly to LTB4 and FMLP. When patients were grouped by disease severity, eosinophil chemotactic responses to PAF were significantly enhanced in severely affected patients (p less than 0.05); this was not true with C5a, LTB4 or FMLP. This enhanced eosinophil chemotaxis to PAF was not related to a specific disease. No correlation between eosinophil chemotactic activity and peripheral blood eosinophil count was observed. The increased responsiveness of circulating eosinophils towards PAF may be related to altered receptor expression during cutaneous inflammation.
我们测定了从炎症性皮肤病患者和健康志愿者中分离出的外周嗜酸性粒细胞（嗜酸性粒细胞）的趋化反应性。研究了10例特应性皮炎患者、5例药物反应患者、10例银屑病患者和14名健康志愿者。使用了特征明确的趋化因子，补体裂解产物C5a、白三烯B4（LTB4）、血小板活化因子（PAF）和N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）作为趋化剂。健康志愿者的嗜酸性粒细胞对C5a和PAF表现出强烈的迁移反应，但对LTB4和FMLP反应较弱。当按疾病严重程度对患者进行分组时，严重受累患者的嗜酸性粒细胞对PAF的趋化反应显著增强（p小于0.05）；C5a、LTB4或FMLP则不然。嗜酸性粒细胞对PAF趋化作用的增强与特定疾病无关。未观察到嗜酸性粒细胞趋化活性与外周血嗜酸性粒细胞计数之间的相关性。循环嗜酸性粒细胞对PAF反应性的增加可能与皮肤炎症期间受体表达的改变有关。
