Czarnetzki B
Clin Exp Immunol. 1983 Nov;54(2):486-92.
In vitro monocyte chemotaxis towards leukotriene B4(LTB4) and platelet activating factor (PAF) was studied with cells from 51 patients with various inflammatory dermatoses and 12 normal volunteers. Monocytes from normal subjects responded poorly to LTB4 (10(-8)-10(-12) M) and PAF (10(-6)-10(-10) M), and cells from patients with urticaria pigmentosa and vericella were even less responsive, while monocytes from patients with severe psoriasis and atopic eczema exhibited markedly enhanced chemotaxis. These changes persisted during high dose therapy with oral steroids, but returned to normal with healing of the skin lesions. Pre-incubation of monocytes with histamine, LTB4, PAF, lymphokines or sera from patients and normal controls did not result in enhanced chemotaxis of the cells. The chemotactic activity of monocytes did not correlate with that of neutrophils in the same patients (r = 0.08). Altered monocyte chemotaxis in patients with inflammatory dermatoses is therefore a reversible process that is related to the severity of the cutaneous inflammation but is not limited to a specific disease.
对51例各种炎症性皮肤病患者及12名正常志愿者的细胞进行体外单核细胞对白三烯B4（LTB4）和血小板活化因子（PAF）的趋化性研究。正常受试者的单核细胞对LTB4（10⁻⁸ - 10⁻¹²M）和PAF（10⁻⁶ - 10⁻¹⁰M）反应较差，色素性荨麻疹和水痘患者的细胞反应更弱，而重度银屑病和特应性皮炎患者的单核细胞趋化性则明显增强。这些变化在口服类固醇高剂量治疗期间持续存在，但随着皮肤病变愈合恢复正常。单核细胞与组胺、LTB4、PAF、细胞因子或患者及正常对照的血清预孵育不会导致细胞趋化性增强。同一患者单核细胞的趋化活性与中性粒细胞的趋化活性不相关（r = 0.08）。因此，炎症性皮肤病患者单核细胞趋化性改变是一个可逆过程，与皮肤炎症的严重程度有关，但不限于特定疾病。
