Aubin F, Blanc D, Guinchard C, Agache P
J Dermatol. 1989 Oct;16(5):369-73. doi: 10.1111/j.1346-8138.1989.tb01282.x.
The authors studied the bioavailability of minocycline in sebum and serum. Blood and sebum samples were collected weekly for 6 weeks from ten healthy volunteers taking 200 mg of minocycline every day for 4 weeks. Sebum was collected by direct extraction with petroleum ether from the forehead. After evaporation, sebum was weighed on a scale accurate to 10 micrograms. Determination of minocycline in serum and sebum was performed using a high performance liquid chromatography technique (HPLC), with a better detection limit at 352 nm than at 400 nm (20 ng/ml and 0.324 microgram/ml respectively). Our results contrast with other studies since no minocycline was detected in the sebum samples of treated subjects and microbiological assays of minocycline in sebum were also negative. In our opinion, the current hypothesis claiming that the effectiveness of minocycline in the treatment of acne vulgaris is based on sebaceous secretion should be reconsidered.
作者研究了米诺环素在皮脂和血清中的生物利用度。从10名健康志愿者中每周采集血液和皮脂样本，持续6周，这些志愿者每天服用200毫克米诺环素，持续4周。通过用石油醚直接从前额提取来收集皮脂。蒸发后，用精确到10微克的天平称量皮脂。使用高效液相色谱技术（HPLC）测定血清和皮脂中的米诺环素，在352纳米处的检测限比在400纳米处更好（分别为20纳克/毫升和0.324微克/毫升）。我们的结果与其他研究形成对比，因为在接受治疗的受试者的皮脂样本中未检测到米诺环素，并且皮脂中米诺环素的微生物检测也为阴性。我们认为，目前声称米诺环素治疗寻常痤疮的有效性基于皮脂分泌的假设应该重新考虑。
