[人增生性瘢痕表皮干细胞用于裸鼠皮肤伤口愈合的实验研究]

[Experimental study of skin wound healing with epidermal stem cells from human hypertrophic scar in nude mice].

作者信息

Zhou Shuping, Huang Zhilong, Ma Jiguang, Xu Jingjing, Cai Jinglong, Zong Xianlei, Du Le

出版信息

Zhonghua Zheng Xing Wai Ke Za Zhi. 2014 Jul;30(4):289-93.

Abstract

OBJECTIVE

To investigate the effect of epidermal stem cells from human hypertrophic scar (HS-ESCs) on the skin wound healing in nude mice.

METHODS

40 mice were randomly divided into two groups as experimental group (n = 20) and control group (n = 20). Wounds, 1 cm in diameters, were made on every mouse back. The wounds were treated with HS-ESCs and erythromycin ointment in experimental group, or only with erythromycin ointment in control group. The wound healing was observed during the following 14 days. The expression of collagen-I, collagen-III, epidermal growth factor (EGF), fibroblast growth factor (FGF2) , transforming growth factor (TGFbeta1, and TGFbeta2) were studied.

RESULTS

The wound healing time in the experimental group was (20.8 +/- 0.84) d, which was (25.6 +/- 0.89) d in the control group. HE staining revealed that the extent of vascularization in the experimental group was 11.60 +/- 0.55, while it was 8.04 +/- 0.33 in the control group. Immunochemistry analysis showed the expression of collagen-I, collagen-III, EGF, FGF2, TGFbeta1, and TGFbeta2 in the experimental group were significantly higher, compared with those in control group (P < 0.05).

CONCLUSION

HS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.

摘要

目的

探讨人增生性瘢痕表皮干细胞（HS-ESCs）对裸鼠皮肤伤口愈合的影响。

方法

将40只小鼠随机分为两组，即实验组（n = 20）和对照组（n = 20）。在每只小鼠背部制作直径为1 cm的伤口。实验组伤口用HS-ESCs和红霉素软膏处理，对照组仅用红霉素软膏处理。在接下来的14天内观察伤口愈合情况。研究I型胶原、III型胶原、表皮生长因子（EGF）、成纤维细胞生长因子（FGF2）、转化生长因子（TGFβ1和TGFβ2）的表达。