Yamane T, Inoue T, Furukawa Y, Hiyoshi M, Sasaki A, Kishida T, Im T, Tatsumi N, Okuda K
Rinsho Byori. 1989 Oct;37(10):1149-54.
Lymphocytes from eight patients with adult T-cell leukemia were analyzed by two color flow cytometry. Monoclonal antibodies (Leu 3 a, Leu 8, Leu 2 a and Leu 15) labelled with fluorescein isothiocyanate or phycoerythrin were used. The purpose was to identify the subsets of the lymphocytes as helper, suppressor/inducer, suppressor or cytotoxic by the surface marker of the cells. All eight patients had antibodies for ATLA. Proviral DNA in the lymphocytes was found in six patients. Summarising the results, OKT4-positive ATL cells were all of the helper T-cell subset, not the inducer subset. OKT8-positive ATL cells were also positive for OKT4 and were all of the cytotoxic T cell subset, not the suppressor subset. In two patients, some ATL cells had both OKT4 and OKT8 on the same cells, especially in the lymph nodes. In our study, ATL cells from eight cases of ATL had all of the helper T subset. These results suggest that the target cells of the human T cell leukemia/lymphoma virus type will be helper T cells.
采用双色流式细胞术分析了8例成人T细胞白血病患者的淋巴细胞。使用了用异硫氰酸荧光素或藻红蛋白标记的单克隆抗体（Leu 3 a、Leu 8、Leu 2 a和Leu 15）。目的是通过细胞的表面标志物将淋巴细胞亚群鉴定为辅助性、抑制/诱导性、抑制性或细胞毒性亚群。所有8例患者均有抗ATLA抗体。6例患者的淋巴细胞中发现了前病毒DNA。总结结果，OKT4阳性的成人T细胞白血病细胞均为辅助性T细胞亚群，而非诱导性亚群。OKT8阳性的成人T细胞白血病细胞OKT4也呈阳性，均为细胞毒性T细胞亚群，而非抑制性亚群。在2例患者中，一些成人T细胞白血病细胞在同一细胞上同时表达OKT4和OKT8，尤其是在淋巴结中。在我们的研究中，8例成人T细胞白血病患者的成人T细胞白血病细胞均有辅助性T亚群。这些结果表明，人类T细胞白血病/淋巴瘤病毒Ⅰ型的靶细胞将是辅助性T细胞。
