Hori Hideo, Iwamoto Ushio, Niimi Gen, Shinzato Masanori, Hiki Yoshiyuki, Tokushima Yasuo, Kawaguchi Kazunori, Ohashi Atsushi, Nakai Shigeru, Yasutake Mikitomo, Kitaguchi Nobuya
Faculty of Clinical Engineering, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
J Artif Organs. 2015 Mar;18(1):55-63. doi: 10.1007/s10047-014-0794-9. Epub 2014 Oct 17.
Scaffolds, growth factors, and cells are three essential components in regenerative medicine. Nonwoven filters, which capture cells, provide a scaffold that localizes and concentrates cells near injured tissues. Further, the cells captured on the filters are expected to serve as a local supply of growth factors. In this study, we investigated the growth factors produced by cells captured on nonwoven filters. Nonwoven filters made of polyethylene terephthalate (PET), biodegradable polylactic acid (PLA), or chitin (1.2-22 μm fiber diameter) were cut out as 13 mm disks and placed into cell-capturing devices. Human mesenchymal stem cells derived from adipose tissues (h-ASCs) and peripheral blood cells (h-PBCs) were captured on the filter and cultured to evaluate growth factor production. The cell-capture rates strongly depended on the fiber diameter and the number of filter disks. Nonwoven filter disks were composed of PET or PLA fibers with fiber diameters of 1.2-1.8 μm captured over 70% of leukocytes or 90% of h-ASCs added. The production of vascular endothelial growth factor (VEGF), transforming growth factor β1, and platelet-derived growth factor AB were significantly enhanced by the h-PBCs captured on PET or PLA filters. h-ASCs on PLA filters showed significantly enhanced production of VEGF. These enhancements varied with the combination of the nonwoven filter and cells. Because of the enhanced growth factor production, the proliferation of human fibroblasts increased in conditioned medium from h-PBCs on PET filters. This device consisting of nonwoven filters and cells should be investigated further for possible use in the regeneration of impaired tissues.
支架、生长因子和细胞是再生医学中的三个基本组成部分。能够捕获细胞的非织造滤器提供了一种支架，可将细胞定位并聚集在受伤组织附近。此外，预计捕获在滤器上的细胞可作为生长因子的局部供应源。在本研究中，我们调查了捕获在非织造滤器上的细胞所产生的生长因子。将由聚对苯二甲酸乙二酯（PET）、可生物降解的聚乳酸（PLA）或几丁质（纤维直径为1.2 - 22μm）制成的非织造滤器切割成13mm的圆盘，并放入细胞捕获装置中。从脂肪组织（h - ASC）和外周血细胞（h - PBC）中提取的人间充质干细胞被捕获在滤器上并进行培养，以评估生长因子的产生情况。细胞捕获率在很大程度上取决于纤维直径和滤器圆盘的数量。由纤维直径为1.2 - 1.8μm的PET或PLA纤维组成的非织造滤器圆盘，能捕获超过70%添加的白细胞或90%的h - ASC。捕获在PET或PLA滤器上的h - PBC可显著增强血管内皮生长因子（VEGF）、转化生长因子β1和血小板衍生生长因子AB的产生。PLA滤器上的h - ASC显示出VEGF的产生显著增强。这些增强作用因非织造滤器和细胞的组合而异。由于生长因子产生的增强，来自PET滤器上h - PBC的条件培养基中人类成纤维细胞的增殖增加。这种由非织造滤器和细胞组成的装置应进一步研究，以探讨其在受损组织再生中的潜在用途。
